Gives access to many free software tools for sequence analysis. EMBOSS aims to serve the molecular biology community. It permits the creation and the release of software in an open source spirit. This tool is useful for sequence analysis into a seamless whole. It is free of charge and is available in open source.
Predicts primer location and polymerase chain reaction (PCR) product sequences from chromosome lists, whole genomes or circular DNA. FastPCR is an integrated tool that finds all possible primer pairs for conventional or multiplex PCR taking into account mismatches located within the specified primer or target sequence. It performs advanced searching for two or more sequences linked to each other and located within a certain distance.
Allows prediction of several kinds of information about an oligonucleotide sequence. OligoAnalyzer is a central calculator enabling comprehensive oligonucleotide analysis. Selection of the analyze button results in the physical properties of the oligonucleotide, such as a complementary sequence, oligonucleotide length, content of G and C bases, melting temperature, extinction coefficient at 260 nm and molecular weight. The software was designed to position locked nucleic acids (LNA) modifications within a sequence, so that the desired melting temperature of the duplex sequence is achieved.
Identifies sequence tagged sites (STSs) within DNA sequences. e-PCR is a web application which searches for sub-sequences that closely match the polymerase chain reaction (PCR) primers and have the correct order, orientation, and spacing. The software uses overlapping discontinuous words to allow matches to be found despite the presence of a mismatch. It also provides a search mode using a query STS against a sequence database.