Primer3 specifications

Information


Unique identifier OMICS_02325
Name Primer3
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages C, Perl
License GNU General Public License version 2.0
Computer skills Advanced
Stability Stable
Maintained Yes

Download


Versioning


Add your version

Documentation


Additional information


http://primer3plus.com/cgi-bin/dev/primer3plus.cgi http://primer3plus.com/primer3web/primer3web_input.htm

Information


Unique identifier OMICS_02325
Name Primer3
Interface Web user interface
Restrictions to use None
Programming languages C, Perl
Computer skills Basic
Stability Stable
Maintained Yes

Documentation


Additional information


http://primer3plus.com/cgi-bin/dev/primer3plus.cgi http://primer3plus.com/primer3web/primer3web_input.htm

Primer3 articles

Primer3 citations

 (36)
2018
PMCID: 5922180

[…] tgc aac agg tga ca-3′] and tet2 [nm_001346736.1] [forward primer 5′-cca cag aga cca gca gaa ca-3′ and reverse primer 5′-tcc gct ttc ttc ttg caa ct-3′] were designed with the primer3 software (http://primer3.ut.ee/). primer's efficiency and specificity was verified using melting curve analysis (single melt curve peak). for the hippocampus, gene expression was normalized to the geometric average […]

2018
PMCID: 5884870

[…] reverse transcribed to synthesize the first-strand cdna using goscript reverse transcription system (promega, fitchburg, ma, usa). all the primers for qrt-pcr were designed in primer 3.0 (http://primer3.ut.ee/) according to the partial sequence obtained by rna-seq (table s3). the qrt-pcr was performed on a stratagene mx3000p thermal cycler (stratagene, la jolla, ca, usa) with an initial […]

2018
PMCID: 5802896

[…] equine centre., the subsequent availability of the ehv-8 strain wh genome sequence and of the orf30 sequence from ehv-8 strain 804/87 [16] allowed sets of overlapping primers to be designed by using primer3 [17], in order to facilitate the amplification and sequencing of the whole of orf30. viral dna from the four viruses was isolated on first passage from the rk13 cell culture supernatant […]

2017
PMCID: 5715621

[…] (invitrogen, usa) following manufacturer’s instructions and using 4 μgr of total rna and random hexamers. gene specific primers were designed for twelve selected degs (table 2) using the primer3 software [88], with a melting temperature between 60 and 65° c, and producing amplicons between 80 and 120 bp. qrt-pcr was performed in a cfx96™ real-time pcr detection system (biorad, usa), […]

2017
PMCID: 5643336

[…] sybr green super mix (invitrogen) and performed in triplicate using the lightcycler 480 (roche diagnostics). primers for amplification were designed in the 3′ region of each gene using the online primer3 tool (http://www.bioinfo.ut.ee/primer3/). the primer sequences are available upon request. amplifications were performed in 20 µl containing 0.5 µm of each primer, 0.5 µl sybr green […]

Primer3 institution(s)
Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Alliance, Heidelberg, Germany

Primer3 reviews

star_border star_border star_border star_border star_border
star star star star star

Be the first to review Primer3