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PrimerBank | A PCR primer database for quantitative gene expression analysis


Provides polymerase chain reaction (PCR) and qPCR primers. PrimerBank contains 497 156 primers that cover 36 928 human and mouse genes, corresponding to around 94% of all known protein-coding gene sequences. These primers have been selected from millions of primer candidates using an improved algorithm and current genomic information. Users can access all stored information (primer sequences and annotations, primer validation data, as well as gene annotations) from the search interface. High-throughput qPCR using PrimerBank primers has several advantages: (i) the primers work under the same conditions and thousands have been experimentally validated and (ii) the expression profiles of thousands of genes can be determined at the same time.

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PrimerBank classification

  • Animals
    • Homo sapiens
    • Mus musculus

PrimerBank specifications

Unique identifier:
Community driven:
User data submission:
Not allowed
Restrictions to use:
Data access:

PrimerBank support



  • Brian Seed <>


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Department of Radiation Oncology, Washington University School of Medicine, Saint Louis, MO, USA; Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA, USA; Department of Genetics, Harvard Medical School, Boston, MA, USA

Funding source(s)

This work was supported by the National Institutes of Health (U01 HL066678 and R01 GM089784).

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