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Publication for PrimerHunter
Environmental DNA (eDNA) Detection Probability Is Influenced by Seasonal Activity of Organisms
[…] Primers for N. alabamensis and S. depressus were designed using PrimerHunter  and checked for cross-amplification with closely related taxa (). Due to the paucity of publicly available sequence data for both the focal Necturus and Sternotherus taxa, fragments fr […]
Environmental DNA (eDNA) detects the invasive rusty crayfish Orconectes rusticus at low abundances
[…] the cytochrome c oxidase subunit 1 (COI) sequences for the 12 species of crayfishes likely to occur in lakes in the upper Midwest, USA (Peters et al. ; Table ), and subsequently designed primers with PrimerHunter (Duitama et al. ). Recommended primer pairs were synthesized by Integrated DNA Technologies (IDT) and evaluated in the laboratory for successful amplification with Orconectes rusticus DNA […]
Neuraminidase Subtyping of Avian Influenza Viruses with PrimerHunter Designed Primers and Quadruplicate Primer Pools
[…] s reported in 2008 to subtype different NA subtypes . Since 2009, several RT-PCR methods were reported for NA gene subtyping [-]. We have previously developed an open source software package called PrimerHunter for the design and selection of virus-subtyping primers. Different from the primer-design software employed by the above reports, PrimerHunter ensures the amplification specificity by acc […]
ecoPrimers: inference of new DNA barcode markers from whole genome sequence analysis
[…] t adapted for long sequences (i.e. whole genomes) and do not take into account the taxonomic discrimination capacity of the amplified sequence during the primer selection process. More interestingly, PrimerHunter () was developed to select highly specific primers for distinguishing virus subtypes, a typical sensu lato barcoding application. Unfortunately, its efficiency on large data sets of long […]
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