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Primerize-2D specifications


Unique identifier OMICS_18272
Name Primerize-2D
Interface Web user interface
Restrictions to use None
Input data Some RNA/DNA sequences.
Output data A text representation of input sequence and target structures aligned, with numbering and chosen mutation range highlighted.
Output format TXT
Programming languages Python
Computer skills Basic
Version 1.6.1
Stability Stable
Source code URL
Maintained Yes



  • person_outline Rhiju Das

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Publication for Primerize-2D

Primerize-2D citation


Allosteric mechanism of the V. vulnificus adenine riboswitch resolved by four dimensional chemical mapping

PMCID: 5847336
PMID: 29446752
DOI: 10.7554/eLife.29602.097

[…] AGACUCCAACAAAAGAAACAACAACAACAAC. Hairpin sequences are in italics; add riboswitch sequence is underlined. The primer assembly scheme and plate orders for all constructs were automatically designed by Primerize-2D ().PCR reactions, including 100 pmol of terminal primers and 1 pmol of internal primers were carried out as previously described. PCR products were purified using Ampure XP magnetic beads […]

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Primerize-2D funding source(s)
Supported by the National Institutes of Health (R01 R01GM102519), a Stanford Graduate Fellowship, and a Burroughs-Wellcome Foundation Career Award at the Scientific Interface.

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