PrimerProspector pipeline

PrimerProspector specifications

Information


Unique identifier OMICS_05884
Name PrimerProspector
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages Python
Computer skills Advanced
Version 1.0.1
Stability Stable
Requirements PyCogent, Numpy, Matpolib
Maintained Yes

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Publication for PrimerProspector

PrimerProspector IN pipeline

2017
PMCID: 5729218
PMID: 29242834
DOI: 10.1128/mSphere.00488-17

[…] to identify pairs with melting temperature differences of <5°c using oligocalc (42). next, the primer pairs were scored for taxonomic coverage and amplicon size using the sis database with primerprospector (39). the amplicon range was chosen to specifically work with the miseq platform and ranged from 358 ± 83 bp to 384 ± 105 bp. finally, the top-performing published primers […]

PrimerProspector institution(s)
Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Boulder, CO, USA; Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO, USA; Cooperative Institute for Research in Environmental Sciences, Boulder, CO, USA; Department of Ecology and Evolutionary Biology, University of Colorado at Boulder, Boulder, CO, USA; Howard Hughes Medical Institute, Boulder, CO, USA
PrimerProspector funding source(s)
Bill and Melinda Gates foundation; Crohn's and Colitis foundation of America; Howard Hughes Medical Institute; National Institutes of Health Signaling and Cell Cycle Regulation Training Grant (T32GM008759)

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