PrimerQuest statistics

Tool stats & trends

Looking to identify usage trends or leading experts?


PrimerQuest specifications


Unique identifier OMICS_15800
Name PrimerQuest
Interface Web user interface
Restrictions to use None
Input data A sequence with length greater than 80 bases. PrimerQuest accepts only nucleic acid bases.
Input format FASTA
Computer skills Basic
Stability Stable
Maintained Yes


  • person_outline PrimerQuest Team

PrimerQuest citations


Minerals in the pre settled coral Stylophora pistillata crystallize via protein and ion changes

Nat Commun
PMCID: 5951882
PMID: 29760444
DOI: 10.1038/s41467-018-04285-7

[…] 0-fold). The transcript levels were determined by using the StepOnePlus System qPCR thermal cycle. Primers specific to CARPs 1–4, STPCA2, and the housekeeping gene actin were designed using the IDT’s PrimerQuest© online tool ( The primer sequences were based on validated sequences of cDNA originating from local S. pistillata RNA,. All reactions containe […]


Evaluation of pre analytical factors affecting plasma DNA analysis

Sci Rep
PMCID: 5943304
PMID: 29743667
DOI: 10.1038/s41598-018-25810-0

[…] sponding probes labeled with FAM as well as 4 long PCR amplicons with mean product size of 471 bp (range 439–522 bp) and corresponding probes labeled with TET (Fig.  and Supplemental Table ). We used PrimerQuest (Integrated DNA Technologies) to design these assays, evaluated them to avoid known polymorphic loci (snapshots from UCSC genome browser indicate common SNPs relative to primer and probe l […]


A genome wide assessment of conserved SNP alleles reveals a panel of regulatory SNPs relevant to the peripheral nerve

BMC Genomics
PMCID: 5930951
PMID: 29716548
DOI: 10.1186/s12864-018-4692-z

[…] s were against the control gene (Gapdh). Probes and primers were ordered from IDT PrimeTime as predesigned assays for rat except for the FAM probe for Aldh5a1, which was custom designed using the IDT PrimerQuest tool with default parameters for two primers and one probe. ddPCRs were set up and run per the manufacturer’s instructions (BioRad), and results were analyzed using QuantaSoft software to […]


NKX2.5 is expressed in papillary thyroid carcinomas and regulates differentiation in thyroid cells

BMC Cancer
PMCID: 5930850
PMID: 29716526
DOI: 10.1186/s12885-018-4399-1

[…] c, Rockford, IL, USA), 0.5 μl specific oligonucleotides (150 nM), 2.5 μl DEPC water and 3 μl diluted cDNA. The oligonucleotides for real-time PCR were purchased from Applied Biosystems, designed with PrimerQuest software (Integrated DNA Technologies, San Diego, CA, USA) and are listed in Additional file . RPL4 was used as internal control. Relative gene expression was determined by subtracting cyc […]


Transcriptome analysis of granulosa cells after conventional vs long FSH induced superstimulation in cattle

BMC Genomics
PMCID: 5902934
PMID: 29661134
DOI: 10.1186/s12864-018-4642-9

[…] en genes (NTS, PTGS2, PTX3, RGS2, INHBA, CCND2 and LRP8) were selected for validation using RT-PCR. Primers were designed using Primer3 (v.0.4.0,, analyzed using IDT PrimerQuest tool (Oligo Analyzer,;, and blasted using NCBI ( […]


An intragenic approach to confer glyphosate resistance in chile (Capsicum annuum) by introducing an in vitro mutagenized chile EPSPS gene encoding for a glyphosate resistant EPSPS protein

PLoS One
PMCID: 5896900
PMID: 29649228
DOI: 10.1371/journal.pone.0194666

[…] cDNA was used for quantitative real-time PCR (qRT-PCR) with SsoAdvanced Universal SYBRGreen Supermix (Bio-Rad, Hercules, CA) in a Bio-Rad CFX Connect Real-Time System. The primers were designed using PrimerQuest Tool (Integrated DNA Technologies, Inc). CaEPSPS-specific primers (forward: 5’-CTG AAG TTG ATG GAG CGA TTT G-3’ and reverse: 5’-CTT TCC CAG GAG ACT TGT ACT T-3’) were used at 375 nM concen […]


Looking to check out a full list of citations?

PrimerQuest reviews

star_border star_border star_border star_border star_border
star star star star star

Be the first to review PrimerQuest