Primers-4-Yeast statistics

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Associated diseases

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Primers-4-Yeast specifications

Information


Unique identifier OMICS_16764
Name Primers-4-Yeast
Interface Web user interface
Restrictions to use None
Input data A gene name list.
Computer skills Basic
Stability Stable
Maintained Yes

Documentation


Maintainer


  • person_outline Maya Schuldiner <>

Publication for Primers-4-Yeast

Primers-4-Yeast in publications

 (3)
PMCID: 5932058
PMID: 29720625
DOI: 10.1038/s41467-018-03957-8

[…] plasmids that are listed in supplementary table . all strains used in this study are listed in supplementary table ., primers for genetic manipulations and their validation were designed using primers-4-yeast (http://wws.weizmann.ac.il/primers-4-yeast). primers for genetic tagging of split venus proteins were designed using primers-4-yeast with flanking sequences primers: […]

PMCID: 5840644
PMID: 29568493
DOI: 10.5256/f1000research.14544.r30437

[…] within the tag sequence) as well as by fluorescent microscopy. primers for creating strains with deletions or c’ tagging of genes (δ mdh1, δ mdh2, δ mdh3 and mdh2-mcherry) were designed using the primers-4-yeast web tool . all deletions were verified using primers from within the endogenous orf. all primers are summarized in ., yeast proteins were extracted by the naoh protocol as previously […]

PMCID: 5748981
PMID: 29187527
DOI: 10.1083/jcb.201704122

[…] manipulated using a transformation method that includes the usage of li-acetate, polyethylene glycol, and single-stranded dna (; ; )., primers for manipulations and validation were designed using primers-4-yeast ()., yeast cells were cultured overnight in synthetic minimal medium (0.67% [wt/vol] yeast nitrogen base with ammonium sulfate, 2% [wt/vol] glucose, amino acid supplements) at 30°c. […]


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Primers-4-Yeast institution(s)
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
Primers-4-Yeast funding source(s)
Supported by funds from the ERC (Grant No. StG 260395) and by the Israeli Ministry of Science.

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