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PrimerSelect specifications

Information


Unique identifier OMICS_26646
Name PrimerSelect
Software type Application/Script
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Maintained No

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No version available

Publication for PrimerSelect

PrimerSelect citations

 (159)
call_split

Elucidating the genetic architecture of reproductive ageing in the Japanese population

2018
Nat Commun
PMCID: 5958096
PMID: 29773799
DOI: 10.1038/s41467-018-04398-z
call_split See protocol

[…] scriptase (Qiagen, Valencia, CA).Relative mRNA abundance was determined using the SYBR GreenER™ qPCR SuperMix system (Invitrogen, Carlsbad, CA). Suitable amplification primers were designed using the PrimerSelect tool of DNASTAR 14 software (Madison, WI) or the NCBI online Primer-Blast program (Supplementary Table ). PCR reactions were performed in a volume of 10 μl containing 1 μl of diluted cDNA […]

call_split

Understanding the ontogeny and succession of Bacillus velezensis and B. subtilis subsp. subtilis by focusing on kimchi fermentation

2018
Sci Rep
PMCID: 5935750
PMID: 29728638
DOI: 10.1038/s41598-018-25514-5
call_split See protocol

[…] icant homology with other B. subtilis group strains were designated PCR targets. The specific primers used for B. velezensis and B. subtilis subsp. subtilis were generated using the DNASTAR Lasergene primerselect module (version 7.0) (Table ). Primer synthesis was performed by the Bioneer Corporation (Daejeon, Korea). Each primer set amplifies a specific DNA fragment from only the targeted species […]

library_books

Identification, Expression, and Regulation of an Omega Class Glutathione S transferase in Rhopalosiphum padi (L.) (Hemiptera: Aphididae) Under Insecticide Stress

2018
Front Physiol
PMCID: 5920109
PMID: 29731722
DOI: 10.3389/fphys.2018.00427

[…] Using the published R. padi transcriptome data (), sense and antisense primers were designed using Lasergene Primerselect (DNASTAR Inc, Madison, WI, United States) to amplify the full-length coding region for the omega GST gene, RpGSTO1. The amplification reaction mix contained 4 mM MgCl2, 100 μM dNTPs, 0.4 […]

library_books

New insight and metrics to understand the ontogeny and succession of Lactobacillus plantarum subsp. plantarum and Lactobacillus plantarum subsp. argentoratensis

2018
Sci Rep
PMCID: 5902611
PMID: 29662105
DOI: 10.1038/s41598-018-24541-6

[…] lantarum and L. plantarum subsp. argentoratensis, were evaluated and confirmed via two-step in-depth testing using the BLAST search engine (https://blast.ncbi.nlm.nih.gov/Blast.cgi) and the Lasergene PrimerSelect program (version 7.2.1; DNASTAR Inc., USA).In L. plantarum subsp. plantarum, the BLASTn searches presented no substantial match to known reference sequences from other Lactobacillus speci […]

call_split

Identification of Candidate Iron Transporters From the ZIP/ZnT Gene Families in the Mosquito Aedes aegypti

2018
Front Physiol
PMCID: 5906682
PMID: 29706902
DOI: 10.3389/fphys.2018.00380
call_split See protocol

[…] al-Time PCR Detection System (Bio Rad), using PerfeCTa qPCR FastMix II (QuantaBio). Primers listed in Supplementary Table were designed using Primer3 server (version 4.0.0) (Untergasser et al., ) or PrimerSelect (DNASTAR), which amplify 107–247 bp fragments of cDNA and amplification efficiency (E) was empirically verified to be 0.9–1.0. Reactions were performed in 15 μL with 200 nM each primer an […]

library_books

Metabolic and Evolutionary Insights in the Transformation of Diphenylamine by a Pseudomonas putida Strain Unravelled by Genomic, Proteomic, and Transcription Analysis

2018
Front Microbiol
PMCID: 5897751
PMID: 29681895
DOI: 10.3389/fmicb.2018.00676

[…] P. putida DPA1 and also for the gyrB gene (used as reference gene in transcription analysis) were manually designed (Supplementary Table ) and checked for the formation of secondary structures by the PrimerSelect™ program (Lasergene®, DNASTAR). The specificity of each primer set was assessed in silico with the online tool Primer-BLAST (http://www.ncbi.nlm.nih.gov/tools/primer-blast/) and by PCR us […]

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PrimerSelect institution(s)
Department of Biochemistry and Molecular Medicine, University of California, School of Medicine, Davis, CA, USA
PrimerSelect funding source(s)
Supported by National Institutes of Health grants HG1736 and AI47400.

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