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PrimerSuite specifications


Unique identifier OMICS_28362
Name PrimerSuite
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes


  • PrimerDimer
  • PrimerPlex
  • PrimerROC


  • person_outline MattTrau <>

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Publications for PrimerSuite

PrimerSuite citations


Exploring target specific primer extension in combination with a bead based suspension array for multiplexed detection and typing using Streptococcus suis as a model pathogen

PMCID: 5753849
PMID: 28980519
DOI: 10.1177/1040638717730384

[…] of the target sequence). the xtag is complementary to the oligonucleotide on the magplex-tag bead (luminex). the boundary between the regions is indicated by a hyphen., tspe primers were designed (primerplex 2.0, premier biosoft) for amplicon production in multiplex pcr reactions (). for all tspe reactions, the primers were designed to hybridize to the sense strand, except for epf (see below). […]


Heterozygosity analysis of polycystic kidney disease 1 gene microsatellite markers for linkage analysis of autosomal dominant polycystic kidney disease type 1 in the Iranian population

PMCID: 5629830
PMID: 29026418
DOI: 10.4103/jrms.JRMS_136_17

[…] kit (genet bio, seoul, south korea) according to the instructions of the manufacturer., the sequences of microsatellite markers were obtained from the ensemble database.[] primers were designed with primerplex 2 v2.5 software (premier biosoft, palo alto ca, usa) for multiplex polymerase chain reaction (pcr) []. primer synthesis and labeling were performed by macrogen (seoul, south korea)., using […]


A Transcriptome Survey Spanning Life Stages and Sexes of the Harlequin Bug, Murgantia histrionica

PMCID: 5492069
PMID: 28587099
DOI: 10.3390/insects8020055

[…] differences was performed on a subset of 16 transcripts using quantitative real-time pcr, incorporating three biological replicates and three technical replicates. primers were designed using primerplex version 2.62 (premier biosoft, palo alto, ca, usa); these data are also presented in . following manufacturer protocol, cdna was synthesized using superscripttm ii reverse transcriptase […]


Molecular diagnostic assays based on cpn60 UT sequences reveal the geographic distribution of subgroup 16SrXIII (A/I)I phytoplasma in Mexico

PMCID: 5430490
PMID: 28424530
DOI: 10.1038/s41598-017-00895-1

[…] been described in detail, . briefly, oligonucleotides that distinguish sbgp/mpv phytoplasma cpn60 ut from other phytoplasma cpn60 ut sequences (supplementary table ) were designed using sigoligo and primerplex v2.62 (premier biosoft, palo alto ca). oligonucleotides were coupled to fluorescent microspheres and hybridized to single-stranded pcr product generated using the cpn60 ut-targeted […]


Evaluation of Different Oligonucleotide Base Substitutions at CpG Binding sites in Multiplex Bisulfite PCR sequencing

PMCID: 5361148
PMID: 28327639
DOI: 10.1038/srep45096

[…] have a cpg in the 5′ end of the forward primer, a cpg in the 5′ end of both the forward and reverse primers, or 0 cpgs in both primers. primers were designed using our custom primer design software primersuite ( with the following parameters: oligo melting temperature (tm) 54 °c, sodium concentration: 50 mm, minimum cpgs in amplicon: 5, minimum number of unconverted […]


A transcriptome profile in hepatocellular carcinomas based on integrated analysis of microarray studies

PMCID: 5237304
PMID: 28086821
DOI: 10.1186/s13000-016-0596-x

[…] rnaeasy mini kit (qiagen, valencia, ca) according to the manufacture’s protocol. ten genes were randomly selected from the 20 most significantly degs. primers for the ten genes were designed using primerplex 2.61 (premier biosoft, palo alto, ca) (additional file : table s1). expression levels of genes were screened by sybr (applied biosystems/life technologies, carlsbad, ca) in abi 7500 real […]

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PrimerSuite institution(s)
Centre for Personalized NanoMedicine, The University of Queensland, St Lucia, QLD, Australian; Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD, Australia; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia
PrimerSuite funding source(s)
Supported by the National Breast Cancer Foundation, Australia [Grant number CG-12-07].

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