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- person_outline MattTrau
Publications for PrimerSuite
Induction of oxidative stress and cell apoptosis by selenium: the cure against oral carcinoma
[…] h PCR product only exhibited one peak. The dissociation curves were analyzed using the Dissociation curve 1.0 Software (Applied Biosystems) for each PCR reaction to detect and eliminate any potential primerdimer formation and nonspecific amplification. The relative abundance of mRNA was calculated according to the method of -2ΔΔCt , accounting for gene-specific efficiencies, and was normalized t […]
Evaluation of Different Oligonucleotide Base Substitutions at CpG Binding sites in Multiplex Bisulfite PCR sequencing
[…] o have a CpG in the 5′ end of the forward primer, a CpG in the 5′ end of both the forward and reverse primers, or 0 CpGs in both primers. Primers were designed using our custom primer design software PrimerSuite (www.primer-suite.com.au) with the following parameters: oligo melting temperature (Tm) 54 °C, sodium concentration: 50 Mm, minimum CpGs in amplicon: 5, minimum number of unconverted bases […]
Better primer design for metagenomics applications by increasing taxonomic distinguishability
[…] are not easily broken, primers hybridize more easily. Furthermore, efficiency of PCR is greatly diminished by allowing forward or reverse primer to either bind to itself (selfDimer) or to each other (primerDimer). It is particularly important that the 3' ends of forward and reverse primers do not create primer dimers. This is implicitly taken care of by the rule of not having more than two strong […]
Desalinated underground seawater of Jeju Island (South Korea) improves lipid metabolism in mice fed diets containing high fat and increases antioxidant potential in t BHP treated HepG2 cells
[…] CTT CCA GCT AGA C-3', reverse 5'- GTC GGC ATG GTC CTG ATT G-3'. The cycling conditions were 95℃ for 10 min, followed by 50 cycles of 95℃ for 10 sec and 60℃ for 1 min. To detect and eliminate possible primerdimer artifacts, the dissociation curve was generated by adding a cycle of 95℃ for 15 sec, 60℃ for 1 min and 95℃ for 15 sec. All primer sets produced amplicons of the expected size and their ide […]
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