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PrimerSuite specifications


Unique identifier OMICS_28362
Name PrimerSuite
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes


  • PrimerDimer
  • PrimerPlex
  • PrimerROC


  • person_outline MattTrau

Additional information

Publications for PrimerSuite

PrimerSuite citations


Induction of oxidative stress and cell apoptosis by selenium: the cure against oral carcinoma

PMCID: 5768350
PMID: 29371933
DOI: 10.18632/oncotarget.22752

[…] h PCR product only exhibited one peak. The dissociation curves were analyzed using the Dissociation curve 1.0 Software (Applied Biosystems) for each PCR reaction to detect and eliminate any potential primerdimer formation and nonspecific amplification. The relative abundance of mRNA was calculated according to the method of -2ΔΔCt [], accounting for gene-specific efficiencies, and was normalized t […]


Evaluation of Different Oligonucleotide Base Substitutions at CpG Binding sites in Multiplex Bisulfite PCR sequencing

Sci Rep
PMCID: 5361148
PMID: 28327639
DOI: 10.1038/srep45096
call_split See protocol

[…] o have a CpG in the 5′ end of the forward primer, a CpG in the 5′ end of both the forward and reverse primers, or 0 CpGs in both primers. Primers were designed using our custom primer design software PrimerSuite ( with the following parameters: oligo melting temperature (Tm) 54 °C, sodium concentration: 50 Mm, minimum CpGs in amplicon: 5, minimum number of unconverted bases […]


Better primer design for metagenomics applications by increasing taxonomic distinguishability

BMC Proc
PMCID: 4044206
PMID: 24564926
DOI: 10.1186/1753-6561-7-S7-S4

[…] are not easily broken, primers hybridize more easily. Furthermore, efficiency of PCR is greatly diminished by allowing forward or reverse primer to either bind to itself (selfDimer) or to each other (primerDimer). It is particularly important that the 3' ends of forward and reverse primers do not create primer dimers. This is implicitly taken care of by the rule of not having more than two strong […]


Desalinated underground seawater of Jeju Island (South Korea) improves lipid metabolism in mice fed diets containing high fat and increases antioxidant potential in t BHP treated HepG2 cells

PMCID: 2830411
PMID: 20198202
DOI: 10.4162/nrp.2010.4.1.3

[…] CTT CCA GCT AGA C-3', reverse 5'- GTC GGC ATG GTC CTG ATT G-3'. The cycling conditions were 95℃ for 10 min, followed by 50 cycles of 95℃ for 10 sec and 60℃ for 1 min. To detect and eliminate possible primerdimer artifacts, the dissociation curve was generated by adding a cycle of 95℃ for 15 sec, 60℃ for 1 min and 95℃ for 15 sec. All primer sets produced amplicons of the expected size and their ide […]


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PrimerSuite institution(s)
Centre for Personalized NanoMedicine, The University of Queensland, St Lucia, QLD, Australian; Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD, Australia; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia
PrimerSuite funding source(s)
Supported by the National Breast Cancer Foundation, Australia [Grant number CG-12-07].

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