PrimerX specifications

Information


Unique identifier OMICS_24986
Name PrimerX
Interface Web user interface
Restrictions to use None
Input data A protein sequence file or a DNA sequence file.
Input format TXT,FASTA
Computer skills Basic
Stability Stable
Maintained Yes

Maintainers


  • person_outline Carlo Lapid <>
  • person_outline Yimin Gao <>

Additional information


https://www.bioinformatics.org/primerx/documentation.html

PrimerX in publications

 (36)
PMCID: 5832746
PMID: 29497119
DOI: 10.1038/s41598-018-22157-4

[…] mutagenesis kit (intron, korea) following the manufacturer’s instructions. complementary mutagenic primers (supplementary data ) used in the mutagenesis were designed using web-based programme primerx (http://www.bioinformatics.org/primerx/). multiple site mutations were carried out one after another by using previously constructed mutant plasmids as templates., prior to electrophoretic […]

PMCID: 5775429
PMID: 29352227
DOI: 10.1038/s41598-018-19790-4

[…] chloride and other chemicals were purchased from sigma-aldrich (milwaukee, wi) unless otherwise indicated in the text. mutagenic primers for site-directed mutagenesis were designed using primerx (http://www.bioinformatics.org/primerx/) and synthesized at integrated dna technologies (coralville, iowa)., for amino acid substitutions within the α4 nachr subunit, point mutations […]

PMCID: 5712788
PMID: 29238341
DOI: 10.3389/fimmu.2017.01607

[…] instruction. the coding sequence of cgdm9cp-1 was confirmed by sequencing, and the plasmid was then transformed into e. coli transetta (de3) cells., the mutagenic primers were designed using the primerx tool (table ). cgdm9cp-1 mutants, including d22a, k43a and h52a, were constructed by pcr amplification. the coding sequence of mutants were cloned and inserted into the pet28a (+) vector […]

PMCID: 5684317
PMID: 29133876
DOI: 10.1038/s41598-017-13071-2

[…] quikchange site-directed mutagenesis kit (agilent) was utilized for generating phenylalanine substitutions for cytoplasmic tyrosine residues. sense and antisense mutant primers were designed using primerx (www.bioinformatics.org/primerx). after amplification with thermal cycling the product was digested with dpn i for 1 hr at 37 °c and then transformed into xl10-gold ultracompetent cells […]

PMCID: 5633207
PMID: 28953979
DOI: 10.1371/journal.ppat.1006589

[…] transformed with the ura5-containing episomal plasmid bearing the wild-type cbp1 gene (pdti22) as previously described []., primers for alanine scanning mutagenesis of cbp1 were designed using primerx (http://www.bioinformatics.org/primerx/). primer sequences are included in supplemental material (). mutations were introduced into pdti22 [] with site-directed mutagenesis using standard […]


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