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Protocols

PrimerZ specifications

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Unique identifier OMICS_02371
Name PrimerZ
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

Publication for PrimerZ

PrimerZ citations

 (10)
library_books

A Children's Oncology Group and TARGET Initiative Exploring the Genetic Landscape of Wilms Tumor

2017
Nat Genet
PMCID: 5712232
PMID: 28825729
DOI: 10.1038/ng.3940

[…] reviewed in igv to confirm their presence., variants of interest in the discovery set that were not verified by the above methods were verified by sanger sequencing. primers were generated with primerz software (http://genepipe.ngc.sinica.edu.tw/primerz/) and pcr amplification was performed using platinum® taq dna polymerase and invitrogen® dntp mix (life technologies corporation, carlsbad, […]

library_books

A MUTYH germline mutation is associated with small intestinal neuroendocrine tumors

2017
Endocr Relat Cancer
PMCID: 5527373
PMID: 28634180
DOI: 10.1530/ERC-17-0196

[…] criteria used for the familial patients., after having identified candidate variants from wgs and wes experiments on familial si-nets cases, we designed validation primers for seven loci using primerz (), using the option ‘input snps or positions’ with default settings except for the product size ranges that was restricted to 100–400 base pairs (http://genepipe.ngc.sinica.edu.tw/primerz/). […]

library_books

A novel splice donor site mutation in EPHA2 caused congenital cataract in a Chinese family

2016
PMCID: 4966373
PMID: 27380975
DOI: 10.4103/0301-4738.185597

[…] sanger sequencing was used to validate the variant identified by wes in the four individuals. polymerase chain reaction (pcr) primers were designed by primer z (http://genepipe.ncgm.sinica.edu.tw/primerz/begindesign.do), the sequences were as follows: 5'-cggcacatagccctcagtaa-3' and 5'-gaggggcagcagtagttaca-3'. after pcr amplification, the purified production was sequenced on abi 3730xl dna […]

library_books

Germline activating MTOR mutation arising through gonadal mosaicism in two brothers with megalencephaly and neurodevelopmental abnormalities

2015
BMC Med Genet
PMCID: 4635597
PMID: 26542245
DOI: 10.1186/s12881-015-0240-8

[…] available family member. target specific primers were designed to include 5′ terminal sense and antisense sequencing tags. the gene-specific moieties of the primers were designed with the aid of primerz []; oligonucleotides were synthesized by integrated dna technologies (coralville, ia) and sequencing was performed on an automated abi3730 dna sequencer (life technologies, carlsbad, ca) […]

call_split

Diagnostic Exome Sequencing Identifies a Novel Gene, EMILIN1, Associated with Autosomal‐Dominant Hereditary Connective Tissue Disease

2015
Hum Mutat
PMCID: 4738430
PMID: 26462740
DOI: 10.1002/humu.22920
call_split See protocol

[…] confirmed using automated fluorescence dideoxy sequencing. cosegregation analysis was performed using the proband, his parents, and his son and daughter. amplification primers were designed using primerz [guenther et al., ]. pcr primers were tagged with established sequencing primers on the 5′ end. sequencing was performed on an abi3730 (life technologies, carlsbad, ca) using standard […]

library_books

Gene–gene interactions between TGF β/Smad3 signalling pathway polymorphisms affect susceptibility to knee osteoarthritis

2015
BMJ Open
PMCID: 4466616
PMID: 26068512
DOI: 10.1136/bmjopen-2015-007931

[…] reaction–restriction fragment length polymorphism (pcr–rflp) analysis. the primer design was based on published sequences or designed using the primer z software (http://genepipe.ngc.sinica.edu.tw/primerz/begindesign.do). pcr cycling conditions were: an initial denaturation at 95°c for 5 min, followed by 35 denaturation cycles at 95°c for 30 s, annealing at 55°c for 30 s, extension at 72°c […]


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PrimerZ institution(s)
National Genotyping Center, Academia Sinica, Taipei, Taiwan, China

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