PrimerZ protocols

View PrimerZ computational protocol

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Associated diseases

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PrimerZ specifications

Information


Unique identifier OMICS_02371
Name PrimerZ
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

Publication for PrimerZ

PrimerZ in pipelines

 (2)
2015
PMCID: 4635597
PMID: 26542245
DOI: 10.1186/s12881-015-0240-8

[…] available family member. target specific primers were designed to include 5′ terminal sense and antisense sequencing tags. the gene-specific moieties of the primers were designed with the aid of primerz []; oligonucleotides were synthesized by integrated dna technologies (coralville, ia) and sequencing was performed on an automated abi3730 dna sequencer (life technologies, carlsbad, ca) […]

2015
PMCID: 4738430
PMID: 26462740
DOI: 10.1002/humu.22920

[…] confirmed using automated fluorescence dideoxy sequencing. cosegregation analysis was performed using the proband, his parents, and his son and daughter. amplification primers were designed using primerz [guenther et al., ]. pcr primers were tagged with established sequencing primers on the 5′ end. sequencing was performed on an abi3730 (life technologies, carlsbad, ca) using standard […]


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PrimerZ in publications

 (9)
PMCID: 5712232
PMID: 28825729
DOI: 10.1038/ng.3940

[…] reviewed in igv to confirm their presence., variants of interest in the discovery set that were not verified by the above methods were verified by sanger sequencing. primers were generated with primerz software (http://genepipe.ngc.sinica.edu.tw/primerz/) and pcr amplification was performed using platinum® taq dna polymerase and invitrogen® dntp mix (life technologies corporation, carlsbad, […]

PMCID: 5527373
PMID: 28634180
DOI: 10.1530/ERC-17-0196

[…] criteria used for the familial patients., after having identified candidate variants from wgs and wes experiments on familial si-nets cases, we designed validation primers for seven loci using primerz (), using the option ‘input snps or positions’ with default settings except for the product size ranges that was restricted to 100–400 base pairs (http://genepipe.ngc.sinica.edu.tw/primerz/). […]

PMCID: 4966373
PMID: 27380975
DOI: 10.4103/0301-4738.185597

[…] sanger sequencing was used to validate the variant identified by wes in the four individuals. polymerase chain reaction (pcr) primers were designed by primer z (http://genepipe.ncgm.sinica.edu.tw/primerz/begindesign.do), the sequences were as follows: 5'-cggcacatagccctcagtaa-3' and 5'-gaggggcagcagtagttaca-3'. after pcr amplification, the purified production was sequenced on abi 3730xl dna […]

PMCID: 4635597
PMID: 26542245
DOI: 10.1186/s12881-015-0240-8

[…] available family member. target specific primers were designed to include 5′ terminal sense and antisense sequencing tags. the gene-specific moieties of the primers were designed with the aid of primerz []; oligonucleotides were synthesized by integrated dna technologies (coralville, ia) and sequencing was performed on an automated abi3730 dna sequencer (life technologies, carlsbad, ca) […]

PMCID: 4466616
PMID: 26068512
DOI: 10.1136/bmjopen-2015-007931

[…] reaction–restriction fragment length polymorphism (pcr–rflp) analysis. the primer design was based on published sequences or designed using the primer z software (http://genepipe.ngc.sinica.edu.tw/primerz/begindesign.do). pcr cycling conditions were: an initial denaturation at 95°c for 5 min, followed by 35 denaturation cycles at 95°c for 30 s, annealing at 55°c for 30 s, extension at 72°c […]


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PrimerZ institution(s)
National Genotyping Center, Academia Sinica, Taipei, Taiwan, China

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