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Primique specifications

Information


Unique identifier OMICS_26648
Name Primique
Interface Web user interface
Restrictions to use None
Programming languages Python
Computer skills Basic
Maintained Yes

Maintainers


  • person_outline Jakob Fredslund
  • person_outline Mette Lange

Publication for Primique

Primique citations

 (7)
library_books

Genome wide identification of wheat (Triticum aestivum) expansins and expansin expression analysis in cold tolerant and cold sensitive wheat cultivars

2018
PLoS One
PMCID: 5875846
PMID: 29596529
DOI: 10.1371/journal.pone.0195138

[…] nts.Due to the high similarity of the homoeologous copies of each gene, the coding domain sequence (CDS) regions of the 45 TaEXPs were used to design for specific primers using Primer 5.0 program and Primique software (http://cgi-www.daimi.au.dk/cgi-chili/primique/front.py) []. Primers were then verified by blasting against genomic sequences in GRAMENE and in EST and NR/NT databases. The β-actin ( […]

library_books

Dominance of P glycoprotein 12 in phenotypic resistance conversion against ivermectin in Caenorhabditis elegans

2018
PLoS One
PMCID: 5825046
PMID: 29474375
DOI: 10.1371/journal.pone.0192995

[…] min, for 40 cycles. Analyses were performed at 7500 Software v2.0.1 and results were determined by using 2-ΔΔCt method []. Specific Pgps and housekeeping primers were designed using a web-based tool (Primique). Primer sequences as well as sequence confirmation PCR products are presented in . […]

library_books

Deciphering the genetic control of fruit texture in apple by multiple family based analysis and genome wide association

2017
J Exp Bot
PMCID: 5441909
PMID: 28338805
DOI: 10.1093/jxb/erx017

[…] an actin gene (MdACT) was employed as housekeeping gene (). For each gene, a pair of discriminant and specific primers was designed (Supplementary Table S5), using Primer3 (http://primer3.ut.ee) and Primique (http://cgi-www.daimi.au.dk/cgi-chili/primique/front.py). […]

library_books

Characteristics and Expression Patterns of the Aldehyde Dehydrogenase (ALDH) Gene Superfamily of Foxtail Millet (Setaria italica L.)

2014
PLoS One
PMCID: 4079696
PMID: 24988301
DOI: 10.1371/journal.pone.0101136

[…] (AF288226.1) was used as the endogenous control; quantification of the 20 SiALDH gene transcript was analyzed using the 2−ΔΔCt method . The primers used for real-time PCR analysis were designed with primique and listed in . The each experiment repeat three times. […]

library_books

Identification of Suitable Reference Genes for Gene Expression Normalization in qRT PCR Analysis in Watermelon

2014
PLoS One
PMCID: 3938773
PMID: 24587403
DOI: 10.1371/journal.pone.0090612

[…] ownloaded from the Pfam database (http://pfam.sanger.ac.uk/) and used as a query against the watermelon proteins (watermelon_v1.pep, ftp://www.icugi.org) using HMMER . The primers were designed using Primique , which can design specific PCR primer pairs for each gene in a family. Specificity of the primers was further checked by electrophoresis in agarose gel and melting curve analysis. Low temper […]

library_books

Screening Suitable Reference Genes for Normalization in Reverse Transcription Quantitative Real Time PCR Analysis in Melon

2014
PLoS One
PMCID: 3903635
PMID: 24475250
DOI: 10.1371/journal.pone.0087197

[…] s downloaded from the Pfam database (http://pfam.sanger.ac.uk/) and used as a query to search against the melon proteins CM_3.5 (https://melonomics.net/) using HMMER . The primers were designed using Primique, which can be used to design the specific PCR primers for each gene in the family . Information regarding the melon catalase family genes and their primers is summarized in . Roots at 0 d, 1 […]

Citations

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Primique institution(s)
Bioinformatics Research Center, University of Aarhus, Århus, Denmark; Research Centre Flakkebjerg, Department of Genetics and Biotechnology, University of Aarhus, Slagelse, Denmark
Primique funding source(s)
Supported by two grants from the Danish Research Council for Technology and Production.

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