PriMux specifications


Unique identifier OMICS_16708
Name PriMux
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes



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  • person_outline David Hysom <>

Publication for PriMux

PriMux in publications

PMCID: 5288812
PMID: 27986722
DOI: 10.1128/AEM.02449-16

[…] sets for each of the 5 organisms and performed a blast search of all primer-probe set sequences against sequence information available for all species within these genera on genbank using the primux software (). this step enabled us to determine that the assays we were developing amplified only targeted genetic material and did not produce a false-positive result when challenged […]

PMCID: 4137498
PMID: 25157264
DOI: 10.1155/2014/101894

[…] a tool for designing multiplex sets of degenerate sequencing primers to tile overlapping amplicons across multiple whole genomes is described. the new script, run_tiled_primers, is part of the primux software. primers were designed for each segment of south american hemorrhagic fever viruses, tick-borne encephalitis, henipaviruses, arenaviruses, filoviruses, crimean-congo hemorrhagic fever […]

PMCID: 3980782
PMID: 24778651
DOI: 10.1155/2014/720585

[…] probes that do not hybridize to any known target sequences and were designed to match the overall gc content and thermodynamics of the target probes., taqman signatures were designed using the run primux triplet script that is part of the primux software [] for the viruses detected in ileum samples 5, 7, 9, 11, and 14. target sets were comprised of the available complete sequences […]

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PriMux institution(s)
Computations, Lawrence Livermore National Laboratory, Livermore, CA, USA; Physics and Life Sciences, Lawrence Livermore National Laboratory, Livermore, CA, USA; Joint Genome Institute, Lawrence Berkeley National Laboratory, Walnut Creek, CA, USA
PriMux funding source(s)
This work was supported by the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52- 07NA27344, by the Laboratory Directed Research and Development grant number 08-SI-002 from Lawrence Livermore National Laboratory and by the Department of Homeland Security, Science and Technology Directorate grant number HSHQDC-07-X-00933.

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