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ProbeMaker specifications


Unique identifier OMICS_00834
Name ProbeMaker
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes


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Publication for ProbeMaker

ProbeMaker in publications

PMCID: 5758537
PMID: 29354053
DOI: 10.3389/fphar.2017.00924

[…] δfosb (cell signaling, 14695s, rabbit), gapdh (millipore, rabbit)., to create our pla probes a rat anti-d1r antibody (sigma, d2944) was conjugated with a plus oligonucleotide (duolink® in situ probemaker plus duo92009, sigma-olink) and a rabbit anti-d2r (millipore, ab5084p) antibody with a minus oligonucleotide (duolink® in situ probemaker minus duo92010, sigma-olink) following […]

PMCID: 5784328
PMID: 29157948
DOI: 10.1016/j.molmet.2017.11.001

[…] factor (ngf) as per the user manual instructions. briefly, the proseek assay is based on proximity extension assay technology. a pair of oligonucleotide labeled antibodies, proseek probes (proseek probemaker a (93001) and proseek probemaker b (93002)), are allowed to bind pair-wise to the target protein present in the sample in a homogeneous assay. when the two proseek probes are in close […]

PMCID: 5561199
PMID: 28819198
DOI: 10.1038/s41598-017-09113-4

[…] with 7.5 μg/ml anti-human ccr7 mab (mab197; r&d systems) or anti-human ccr1 mab (clone 53504; r&d systems) conjugated with either the plaminus or plaplus probes generated with duolink ii probemaker (sigma). the images were acquired using a confocal laser microscope (fv1000, fv1000-d or lsm710) or a fluorescence microscope (bx50), and dimerized ccr7 observed as orange dots […]

PMCID: 5540560
PMID: 28764767
DOI: 10.1186/s13195-017-0279-1

[…] five times each for 2 min with wash buffer a (olink bioscience). next, the cells were incubated with pla probes made in our laboratory from streptavidin and rabbit anti-mao-b igg (dsp) by duolink probemaker kits minus and plus (olink bioscience), respectively, for 1 h at 37 °c, followed by washing five times each for 2 min with wash buffer a. for each experimental condition, we made two […]

PMCID: 5641116
PMID: 29069773
DOI: 10.18632/oncotarget.19552

[…] in phosphate-buffered saline (pbs) and permeabilized for 15 min with 0.2% triton x-100 in pbs. an in situ pla for detection of egfr homodimers was performed with duolink ii pla probes, probemaker, and detection reagents (olink bioscience). rabbit monoclonal antibodies to egfr (abcam) were conjugated to plus or minus pla oligonucleotide arms with the use of probemaker. cells grown […]

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ProbeMaker institution(s)
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden

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