Main logo
tutorial arrow
Create your own tool library
Bookmark tools and put favorites into folders to find them easily.

ProDesign | An approach to oligonucleotide probe design for genomes and gene families


A tool developed for the selection of oligonucleotide probes to detect members of gene families present in environmental samples. Gene family-specific probe sequences are generated based on specific and shared words, which are found with the spaced seed hashing algorithm. To detect more sequences, those sharing some common words are re-clustered into new families, then probes specific for the new families are generated. ProDesign is very flexible in that it can be used for designing probes for detecting many genes families simultaneously and specifically in one or more genomes. Neither the length nor the melting temperature of the probes needs to be predefined. The program provides biologists with a powerful tool for an easy, rapid and flexible design of oligonucleotide probes for environmental microarrays and related applications.

User report

tutorial arrow
Vote up tools and offer feedback
Give value to tools and make your expertise visible
Give your feedback on this tool
Sign up for free to join and share with the community

0 user reviews

star_border star_border star_border star_border star_border
star star star star star

0 user reviews

star_border star_border star_border star_border star_border
star star star star star

No review has been posted.

ProDesign forum

tutorial arrow
Communicate with other users
Participate in the forum to get support for using tools. Ask questions about technical specifications.
Take part in the discussion
Sign up for free to ask question and share your advices

No open topic.

ProDesign classification

ProDesign specifications

Unique identifier:
Restrictions to use:
Computer skills:
Web user interface
Input data:
Clusters of homologous sequences


tutorial arrow
Promote your skills
Define all the tasks you managed and assign your profile the appropriate badges. Become an active member.
Promote your work
Sign up for free to badge your contributorship



Institute of Computing Technology, Chinese Academy of Sciences, China; Ontario Cancer Institute, University Health Network, Toronto, Canada; Department of Medical Biophysics, University of Toronto, Canada

Funding source(s)

Grants NSFC 60372040 and NSFC 90612019, State Scholarship Fund Award of China, and a CIHR grant

By using OMICtools you acknowledge that you have read and accepted the terms of the end user license agreement.