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ProMoST specifications


Unique identifier OMICS_10653
Name ProMoST
Interface Web user interface
Restrictions to use None
Input data Protein sequence
Computer skills Basic
Stability Stable
Maintained Yes


  • person_outline Brian Halligan <>

Publications for ProMoST

ProMoST in publications

PMCID: 5260966
PMID: 28248243
DOI: 10.3390/proteomes4040033

[…] from normal and cancer tissues []. the pi/mw parameters can be computed not only for a polypeptide’s canonical (nonmodified) sequences but also for post-translationally modified forms as well. the promost program can calculate pi/mw taking into account such modifications as phosphorylation (presence of phosphoserine/threonine (s/t), phosphotyrosine (y)), deamidation of asparagine (n) […]

PMCID: 5050428
PMID: 27703170
DOI: 10.1038/srep33954

[…] to the larger 29 kda band. the theoretical pi values for ca18 and ca19 were estimated at 5.51 and 6.51, respectively, with a prediction of 20 and 19 probable modifications by phosphorylation each (promost. ), with predicted pi values ranging from 4.64–5.39 and 5.05–6.27. the netphos 3.1 server analysis predicted 27 and 34 potential phosphorylation sites for ca18 and ca19, respectively (; ). […]

PMCID: 4673442
PMID: 26682052
DOI: 10.1038/cti.2015.22

[…] six phosphoforms of lpap that we detected on 2d-page., our experimental results have shown that lpap proteoforms from the upper train detected on 2d-page have pi values of 4.54, 4.47 and 4.40. using promost software, we calculated that unphosphorylated, mono-, di- and tri-phosphorylated forms of lpap should have pi values of ~4.40, 4.34, 4.28 and 4.22, respectively. these estimates were slightly […]

PMCID: 3996944
PMID: 24735559
DOI: 10.1186/1559-0275-11-16

[…] of cell biology, disease treatment, and disease prevention. 2-de provides a direct observation of protein ptms in gels as well as its relative abundance. publicly available web-based tools such as promost [] and jvirgel [] could be used for that purpose. in addition, specific staining dyes for ptm monitoring were developed and became widely applied in the proteome studies. in phosphoproteome […]

PMCID: 2739297
PMID: 19759909
DOI: 10.1371/journal.pone.0006964

[…] be oxidized to cysteic acid . this conversion adds a negative charge to the protein and may explain why peroxiredoxin was in multiple spots. interestingly, analysis of sso2121 peroxiredoxin using promost (protein modification screening tool; indicates that modification of one cysteine to cysteic acid would shift the position horizontally one ph unit. […]

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ProMoST institution(s)
Bioinformatics Research Center, Medical College of Wisconsin, Milwaukee, WI, USA; Biotechnology and Biomedical Engineering Center, Medical College of Wisconsin, Milwaukee, WI, USA; Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, USA

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