ProSight Lite protocols

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ProSight Lite specifications


Unique identifier OMICS_09678
Name ProSight Lite
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Windows
Computer skills Medium
Stability Stable
Maintained Yes


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  • person_outline Paul M. Thomas <>

Publication for ProSight Lite

ProSight Lite in pipeline

PMCID: 5647560
PMID: 28938074
DOI: 10.1021/acs.analchem.7b01581

[…] sites 6–79, and one at lower energy for fragments from sites 114–130. data analysis was performed with xtract (thermo scientific) or mmass software, and graphical fragment maps were created with prosight lite. all intensity values used for the determination of weighted-average charges were normalized by charge to account for the detection bias in the orbitrap mass analyzer., front-end […]

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ProSight Lite in publications

PMCID: 5912465
PMID: 29487134
DOI: 10.1074/jbc.RA117.000487

[…] threshold of 3. the xtract algorithm from xcalibur 3.0 software (thermo fisher scientific) was used to determine the decharged masses of the observed ions, which were then inserted into prosight lite ( for identification of putative post-translational modifications within a precursor and product ion tolerance of 10 ppm. aliquots of ccl8 […]

PMCID: 5799867
PMID: 29249155
DOI: 10.1021/acs.jproteome.7b00622

[…] deconvolution 4.0 (thermo fisher scientific, bremen, germany). deconvolution settings were s/n threshold: 1, fit factor: 80%, and remainder threshold: 25%. deconvoluted results were searched using prosight lite. ion types matched were b, y, c, and z with a 10 ppm mass error limit., the ecd fragmentation spectrum of sp [m + 2h]2+ ion (a) demonstrates the advantages of the design […]

PMCID: 5750139
PMID: 29172454
DOI: 10.1021/acs.analchem.7b04810

[…] etd spectra for both n- and o-glycopeptides., informatics tools for analysis of etd-based top-down proteomic data continue to improve as well. kelleher et al. launched the freely available prosight lite in 2015, which is a simple and intuitive platform to characterize proteoforms based on ms/ms spectra. prosight lite is compatible with a range of dissociation methods, including etd, […]

PMCID: 4767540
PMID: 26780093
DOI: 10.1038/nmeth.3731

[…] ms2 and ms3 results were then subjected to a database search against the constructed corum-proteoform database () using absolute mass mode. all masses used for the identifications can be found in . prosight lite was used to visualize the fragmentation of identified proteins and produce graphical fragment maps., similarly to the recent c-score approach for the characterization of proteoforms, […]

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ProSight Lite institution(s)
Department of Chemistry, Department of Molecular Biosciences, Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA

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