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ProSight Lite specifications


Unique identifier OMICS_09678
Name ProSight Lite
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Windows
Computer skills Medium
Stability Stable
Maintained Yes


No version available


  • person_outline Paul M. Thomas

Publication for ProSight Lite

ProSight Lite citations


The N terminal domain of a tick evasin is critical for chemokine binding and neutralization and confers specific binding activity to other evasins

PMCID: 5912465
PMID: 29487134
DOI: 10.1074/jbc.RA117.000487

[…] gnal-to-noise threshold of 3. The Xtract algorithm from Xcalibur 3.0 software (Thermo Fisher Scientific) was used to determine the decharged masses of the observed ions, which were then inserted into ProSight Lite ( for identification of putative post-translational modifications within a precursor and product ion tolerance of 10 ppm. Aliquots of CCL8 and degly […]


Exploring ECD on a Benchtop Q Exactive Orbitrap Mass Spectrometer

J Proteome Res
PMCID: 5799867
PMID: 29249155
DOI: 10.1021/acs.jproteome.7b00622
call_split See protocol

[…] in Deconvolution 4.0 (Thermo Fisher Scientific, Bremen, Germany). Deconvolution settings were S/N Threshold: 1, Fit Factor: 80%, and Remainder Threshold: 25%. Deconvoluted results were searched using ProSight Lite. Ion types matched were b, y, c, and z with a 10 ppm mass error limit. […]


Native Electron Capture Dissociation Maps to Iron Binding Channels in Horse Spleen Ferritin

Anal Chem
PMCID: 5647560
PMID: 28938074
DOI: 10.1021/acs.analchem.7b01581
call_split See protocol

[…] from sites 6–79, and one at lower energy for fragments from sites 114–130. Data analysis was performed with Xtract (Thermo Scientific) or mMass software, and graphical fragment maps were created with Prosight Lite. All intensity values used for the determination of weighted-average charges were normalized by charge to account for the detection bias in the Orbitrap mass analyzer.Front-end infrared […]


An informatic framework for decoding protein complexes by top down mass spectrometry

Nat Methods
PMCID: 4767540
PMID: 26780093
DOI: 10.1038/nmeth.3731
call_split See protocol

[…] . MS2 and MS3 results were then subjected to a database search against the constructed CORUM-Proteoform database () using absolute mass mode. All masses used for the identifications can be found in . ProSight Lite was used to visualize the fragmentation of identified proteins and produce graphical fragment maps. […]


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ProSight Lite institution(s)
Department of Chemistry, Department of Molecular Biosciences, Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA

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