A method for non-specifically capturing cross-linked chromatin complexes via protein carboxylate groups that allows the DNA to be subjected to all downstream chemical treatments. PAtCh-Cap is designed to be facile and universally applicable to any of the current and future ChIP-based techniques that perform additional chemical and library preparation steps on bead-bound chromatin.
Department of Chemistry, University of Utah, Salt Lake City, UT, USA
PAtCh-Cap funding source(s)
This work was supported by the Department of Chemistry at the University of Utah, the American Cancer Society (grant N°RSG- 14-185-01-DMC) and the National Cancer Institute for the Huntsman Cancer Institute core facilities (grant n°P30CA042014).