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Protein Thermal Shift Software specifications


Unique identifier OMICS_06035
Name Protein Thermal Shift Software
Software type Toolkit/Suite
Interface Command line interface
Restrictions to use License purchase required
Operating system Unix/Linux
License Commercial
Computer skills Advanced
Stability Stable
Maintained Yes


No version available

Protein Thermal Shift Software citations


Using Microscale Thermophoresis to Characterize Hits from High Throughput Screening: A European Lead Factory Perspective

SLAS Discov
PMCID: 5824829
PMID: 29460707
DOI: 10.1177/2472555217744728

[…] th increments of 0.05 °C per second, a scan rate selected to maximize screening efficiency while not adversely affecting the quality of the data. Data were analyzed using the derivative method in the Protein Thermal Shift software from the QuantStudio 5 instrument (Applied Biosystems). A difference in target protein melting temperature in the presence of compound (ΔTm) of >1.5 °C was considered si […]


A secondary RET mutation in the activation loop conferring resistance to vandetanib

Nat Commun
PMCID: 5809600
PMID: 29434222
DOI: 10.1038/s41467-018-02994-7

[…] es) was added to each drug treatment, and the thermal shift was measured in a 7500 Fast RT-PCR machine (Applied Biosystems) in a temperature range of 25–90 °C. Subsequent analysis was performed using Protein Thermal Shift Software v1.2 (Applied Biosystems). […]


A conformational switch high throughput screening assay and allosteric inhibition of the flavivirus NS2B NS3 protease

PLoS Pathog
PMCID: 5462475
PMID: 28542603
DOI: 10.1371/journal.ppat.1006411

[…] phobic regions of the denatured protein. The melting temperature (Tm) was calculated as the mid-log of the transition phase from the native to the denatured protein using a Derivative model using the Protein Thermal Shift Software v1.0 (ThermalFisher Scientific). The reference unfolding temperature of proteins in 1.6% DMSO (Tm-DMSO) was subtracted from the values in the presence of each compounds […]


Optimization of the Production Process and Characterization of the Yeast Expressed SARS CoV Recombinant Receptor Binding Domain (RBD219 N1), a SARS Vaccine Candidate

PMCID: 5612335
PMID: 28456726
DOI: 10.1016/j.xphs.2017.04.037

[…] ermal shift assay was then performed by heating the sample from 25°C to 95°C and monitoring the fluorescence intensity change using Protein Thermal Shift™ reagents, a ViiA™ 7 Real-Time PCR system and Protein Thermal Shift™ software version 2.0. […]


Icaritin enhances mESC self renewal through upregulating core pluripotency transcription factors mediated by ERα

Sci Rep
PMCID: 5238509
PMID: 28091581
DOI: 10.1038/srep40894

[…] epeats. Protein melt reactions (25 °C for 2 min at 1.6 °C/s and 99 °C for 2 min at 0.05 °C/s) were run in ViiA 7 real-time PCR system. The resulting melt curves and temperature shift were analyzed by Protein Thermal Shift Software v1.2 (Invitrogen). […]


Successful engineering of a highly potent single chain variable fragment (scFv) bispecific antibody to target disialoganglioside (GD2) positive tumors

PMCID: 4938304
PMID: 27471647
DOI: 10.1080/2162402X.2016.1168557

[…] ed with Protein Thermal Shift dye and fluorescence was monitored using a StepOnePlus quantitative PCR machine (Applied Biosystems) with a 1% thermal gradient from 25 to 99°C. Data were analyzed using Protein Thermal Shift Software (Applied Biosystems) to calculate the Tm. A single domain hu3F8-scFv construct was analyzed and used to appropriately assign the correct peaks for the hu3F8-scFv and huO […]


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Protein Thermal Shift Software institution(s)
ThermoFisher Scientific

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