Protein Thermal Shift Software statistics

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Protein Thermal Shift Software specifications

Information


Unique identifier OMICS_06035
Name Protein Thermal Shift Software
Software type Toolkit/Suite
Interface Command line interface
Restrictions to use License purchase required
Operating system Unix/Linux
License Commercial
Computer skills Advanced
Stability Stable
Maintained Yes

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Protein Thermal Shift Software in publications

 (20)
PMCID: 5923107
PMID: 29507069
DOI: 10.1128/AAC.02269-17

[…] dye (thermo fisher scientific, waltham, ma). melting temperatures were determined by curve fitting the first derivative of the fluorescence data in microsoft excel software and confirmed, using protein thermal shift software (version 1.0; life technologies, carlsbad, ca), using the derivative tm method. all plots were made using prism software (graphpad software, inc., la jolla, ca). tm […]

PMCID: 5809600
PMID: 29434222
DOI: 10.1038/s41467-018-02994-7

[…] was added to each drug treatment, and the thermal shift was measured in a 7500 fast rt-pcr machine (applied biosystems) in a temperature range of 25–90 °c. subsequent analysis was performed using protein thermal shift software v1.2 (applied biosystems)., the time course of autophosphorylation of recombinant purified ret kd (2.5 μm) was examined in the presence of saturating concentrations […]

PMCID: 5577305
PMID: 28855508
DOI: 10.1038/s41467-017-00444-4

[…] using xlfit (idbs, london, uk). alternatively, melting curves were recorded between 10 and 70 °c with the quantstudio7 flex qpcr machine (life technologies, carlsbad, ca, usa) and analyzed using the protein thermal shift software v1.0 (applied biosystems, ny, usa). in both cases the temperature ramp rate was 0.017 °c s−1 and data were fitted with a boltzmann sigmoidal function., following […]

PMCID: 5548783
PMID: 28790404
DOI: 10.1038/s41598-017-07755-y

[…] gradient from 25 to 95 °c at a constant rate of 0.05 °c/sec and protein melting temperatures were calculated based on a boltzmann function fitting to experimental data, as implemented in the protein thermal shift software (applied biosystems). protein in 3.1% dmso was used as a reference., pdb accession codes are 3op5 (vrk13-364/asc24), 5uu1 (vrk214-335/bi-d1870); 5uvf […]

PMCID: 5445127
PMID: 28603531
DOI: 10.3389/fpls.2017.00852

[…] temperature gradient from 25 to 95°c at a constant rate of 0.05°c/s and protein melting temperatures were calculated based on a boltzmann function fitting to experimental data, as implemented in the protein thermal shift software (applied biosystems, singapore). protein in 0.1% dmso was used as a reference. compounds displaying a positive temperature shift (δtm) of 2°c or higher compared […]


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