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protk specifications

Information


Unique identifier OMICS_24281
Name protk
Alternative name Proteomics toolkit
Software type Toolkit/Suite
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Ruby
Computer skills Advanced
Stability Stable
Maintained Yes

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Maintainer


  • person_outline Ira Cooke <>

protk in publications

 (34)
PMCID: 5876329
PMID: 29599527
DOI: 10.1038/s41598-018-23690-y

[…] 2, the predicted function of exd2 combined with a mitochondrial localization suggested it might be involved in mtdna metabolism. examination of exd2 mitochondrial localization using proteinase k (protk) protection showed that the protein was sensitive to digestion using intact mitochondria and outer-membrane-lysed mitochondria (here referred to as mitoplasts, see), without the addition […]

PMCID: 5856005
PMID: 29568513
DOI: 10.1186/s13630-018-0056-1

[…] on a zeiss lsm5 pascal confocal microscope system using a water-dipping lens via sequential laser excitation., whole mount in situ hybridization was performed as previously described [], without the protk treatment to preserve the superficial localized olfactory epithelium. the omp probe was produced as described []. stained, 4% pfa fixed embryos were transferred into pbs:glycerol (1:1) […]

PMCID: 5816147
PMID: 29378946
DOI: 10.1073/pnas.1711837115

[…] after 60 min with (l)-glp1., resistance to protease degradation is one of the most useful properties of d-peptides generally. we carried out quantitative analysis of the (d)-glp1 proteinase k (protk) resistance and compared with (l)-glp1. shows total loss of (l)-glp1 in <1 h, while 80% of (d)-glp1 can still be detected after 6 h exposure to protk., to demonstrate the general […]

PMCID: 5778363
PMID: 29158431
DOI: 10.1128/IAI.00386-17

[…] properties. exosomes were lysed by using heat to release their content, similarly to a method described previously (). intact and heat-lysed exosomes were then treated with proteinase k (protk) to cleave and inactivate any protein component of vesicles. intact exosomes, protk-treated exosomes, and lysed exosomes as well as lysed exosomes treated with protk were used to treat naive […]

PMCID: 5762901
PMID: 29321583
DOI: 10.1038/s41598-017-18649-4

[…] protein g (thermofisher) and 1 ug h3k4me3 rabbit polyclonal antibody (diagenode). chromatin was eluted in 1% sds, and 0.1 m nahco3, de-crosslinked at 65 °c for 8–12 hours, treated with rnase a and protk, and purified using phenol/chloroform extraction. mouse insulin promoter primers and myl6 primers were used as negative control and positive/normalization control, respectively. for 3c: […]


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