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Protocol Exchange specifications


Unique identifier OMICS_18313
Name Protocol Exchange
Alternative name Protocol Exchange from Nature Research
Restrictions to use None
Community driven Yes
Data access Browse
User data submission Allowed
Content license Other
Maintained No


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Publication for Protocol Exchange from Nature Research

Protocol Exchange citations


miR200 regulated CXCL12β promotes fibroblast heterogeneity and immunosuppression in ovarian cancers

Nat Commun
PMCID: 5849633
PMID: 29535360
DOI: 10.1038/s41467-018-03348-z

[…] for references of each antibody and Supplementary Fig. , for detailed description of the gating strategy).The Treg suppressive assay was adapted from ref. , Benoit Salomon’s lab protocol available on protocol exchange. CD4+CD25+ were isolated from healthy donor PBMCs as described earlier. The negative fraction (CD4+CD25−cells containing T effector cells) was also recovered and kept overnight at 4  […]


Achieving better than 3 Å resolution by single particle cryo EM at 200 keV

Nat Methods
PMCID: 5679434
PMID: 28991891
DOI: 10.1038/nmeth.4461
call_split See protocol

[…] ing aldolase imaging, adjustments to spot size were made and alignments were repeated, including maximization of parallel illumination. Coma-free alignment was then performed as described previously (Protocol Exchange DOI). Daily adjustments, if necessary, were made during data collection to maintain lens stigmation and ensure the beam was coma-free.All cryo-EM data were acquired using the Leginon […]


Altered Prostanoid Metabolism Contributes to Impaired Angiogenesis in Persistent Pulmonary Hypertension of the Newborn

PMCID: 4346417
PMID: 25521916
DOI: 10.1038/pr.2014.209

[…] . Complementary DNA was synthesized from the extracted RNA using the iScript cDNA synthesis kit (Bio-Rad). The PCR primers were designed using Primer3 and primer sequences are provided online through protocol exchange. Real time RT-PCR was performed using the iQ5 multicolor real-time PCR detection system (Bio-Rad). The PCR cycle started at 95°C for 3 min followed by 40 cycles of 95°C for 15 s and […]


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