PSF Lab specifications


Unique identifier OMICS_28705
Name PSF Lab
Software type Application/Script
Interface Graphical user interface
Restrictions to use Academic or non-commercial use
Operating system Mac OS, Windows
Computer skills Medium
Version 3.0
Stability Stable
Maintained Yes


No version available



  • person_outline Jorg Woehl

Publication for PSF Lab

PSF Lab citations


Persistent microglial activation and synaptic loss with behavioral abnormalities in mouse offspring exposed to CASPR2 antibodies in utero

Acta Neuropathol
PMCID: 5587616
PMID: 28755208
DOI: 10.1007/s00401-017-1751-5

[…] he WPL deconvolution algorithm in the Parallel Iterative Deconvolution ImageJ plugin [] based on the Iterative Deconvolve 3D algorithm [] utilizing a theoretical 3D point spread function generated in PSF Lab []. Image stacks were then filtered with a 3 × 3 median filter and thresholded using the OTSU method [] to obtain accurate binary representations of positive profiles. To assess synapse profil […]


Single molecule tracking in live cells reveals distinct target search strategies of transcription factors in the nucleus

PMCID: 4095940
PMID: 24925319
DOI: 10.7554/eLife.02230.028

[…] The PSF of the single particle signal was obtained with the PSF Lab software (). The parameters used to retrieve the PSF were: emission wavelength 600 nm, objective NA 1.49, coverslip thickness 150 μm, oil refractive index 1.51, coverslip refractive index 1.52 […]

PSF Lab institution(s)
Department of Chemistry and Biochemistry, Laboratory for Surface Studies, University of Wisconsin-Milwaukee, Milwaukee, WI, USA

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