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Associated diseases

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PUMA2 specifications

Information


Unique identifier OMICS_04580
Name PUMA2
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability No
Maintained No

Publication for PUMA2

PUMA2 in publications

 (10)
PMCID: 5832149
PMID: 29483520
DOI: 10.1038/s41598-018-21628-y

[…] of ab31 num1:tdeosfp., ab31 num1: tdeosfp∆kin1; ab31 num1:tdeosfp∆kin3:: the open reading frame of kin1 or kin3 was replaced in ab31 num1:tdeosfp by the nourseothricin resistance cassette from puma262 using the pcr-based protocol for gene replacements previously described. flanking regions for deletion of kin1 (umag_04218; genbank xp_011390699.1) were generated by pcr using primer pairs […]

PMCID: 5630700
PMID: 29038656
DOI: 10.3389/fimmu.2017.01234

[…] change mutagenesis (stratagene) and synthetic dna encoding a portion of the hcmv promoter and intron a was mobilized from puc57 cmv-inta via snabi and nhei to replace the resident 5′-ntr to create puma2. previously described human codon-optimized genes residing in pcdnasfi encoding marburg musoke (marv), ebola zaire kikwit (ebov), or ebola bundibugyo np were back inserted to puma2 via sfii. […]

PMCID: 5623874
PMID: 29021793
DOI: 10.3389/fimmu.2017.01197

[…] genes residing in pcdnasfi and encoding marburg musoke np, ebola zaire kikwit, and bundibugyo np () (genbank accession numbers mf871598, mf871593, and mf871595, respectively) were subcloned to puma2 (pcdnasfincoi− with the cmv intron a replacing the tripartite leader and murine splicing region) to generate elisa substrates (see below). the marburg and zaire versions were also pcr amplified […]

PMCID: 5454850
PMID: 28468279
DOI: 10.3390/ijms18050937

[…] []. homologous single or multiple integrations at the ip locus were verified by southern blot analysis using a 2.1-kb probe obtained with the primer combination omf502 × omf503 () and the template puma260 [,]., to generate plasmid prabx2 pomahis-αgfpnb-th-cts1 (puma2240) the sequence of the llama-derived αgfp nanobody [] was dicodon-optimized () and synthetized by the company geneart (thermo […]

PMCID: 5342259
PMID: 28273139
DOI: 10.1371/journal.pone.0173389

[…] of the optical density at 600 nm., escherichia coli top10 (invitrogen/life technologies) was used for cloning purposes with conventional culture and transformation techniques []. plasmid puma2163 was generated using the golden gate cloning technology []. briefly, upstream flank (uf) and downstream flank (df) of aox1 (accession number umag_02774) were generated by pcr on um521 genomic […]


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PUMA2 institution(s)
Mathematics and Computer Science Division, Argonne National Laboratory Argonne, IL, USA; Computation Institute, University of Chicago Chicago, IL, USA; University of Illinois at Chicago Chicago, IL, USA

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