PyroBayes protocols

PyroBayes computational protocol

PyroBayes specifications


Unique identifier OMICS_01155
Name PyroBayes
Software type Application/Script
Interface Command line interface
Restrictions to use Academic or non-commercial use
Biological technology Roche
Operating system Unix/Linux
Computer skills Advanced
Version 0.9
Stability Stable
Maintained Yes


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  • person_outline Aaron Quinlan <>
  • person_outline Gabor Marth <>

Publication for PyroBayes

PyroBayes IN pipelines

PMCID: 4060450
PMID: 24886912
DOI: 10.1186/ar4566

[…] in the 454 data. capillary sequencing of these amplicons used the 3730xl platform (applied biosystems, ca, usa)., the 454 sequence read demultiplexing used ”untag” software [24]; base calling used pyrobayes [25]; and alignment to the reference genome used bwa [26]. variant calling was carried out by using gatk [27] without duplicate read removal, by using hard filters: coverage at least […]

PMCID: 3526165
PMID: 23077218
DOI: 10.1093/hmg/dds435

[…] 10 s and 70°c for 4 s. empcr and bidirectional sequencing were performed according to the manufacturer's instructions (roche 454)., base calling and alignment were performed using the algorithm of pyrobayes and mosaik. subsequent analysis of variants was performed using the custom r library flowgram. to maximize specificity for very low-level variants, base positions within or immediately […]

PyroBayes institution(s)
Department of Biology, Boston College, Chestnut Hill, MA, USA
PyroBayes funding source(s)
Supported by a grant from the US National Human Genome Research Institute (R01 HG003698).

PyroBayes review

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Hector Snf

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Great caller