pySolo

[…] monitored during this period, and data from the second day of the dark/dark phase were used for calculations. activity was measured in 1-min bins using software provided by the manufacturer, and the pysolo software () was used to quantify fly sleep. inactivity of a fly for 5 min or more was considered as sleep. the flies received normal food (5% sucrose in 1% agarose) prior to their individual […]

[…] during the experiment, flies were supplemented with agar food (2% agar and 5% sucrose). the sleep assay was performed at fixed humidity and temperature (25 ± 1°c). data were collected according to pysolo manual and analyzed via pysolo sleep analysis software[]., all raw sequencing data are available for download from national center for biotechnology information geo database under accession […]

[…] flies were placed individually in trikinetics glass tubes, video monitored under infra-red illumination during 3 days in ld, then placed for at least 5 days in ll. the images were processed using pysolo video software [] to determine the distance travelled (in pixels) for each minute of the day. sleep was defined as 5 min of more of immobility []. at least 40 flies in 2–3 replicates […]

[…] (tritech research). data were collected in 1-min bins, and a sliding window was applied. sleep was defined as 5 consecutive minutes of inactivity as described previously. data were analyzed with pysolo software. for analysis of circadian behavior, locomotor activity was measured using dam2 monitors (trikinetics), and activity counts were collected in 30 min bins in ld for 4 days, […]

[…] at the end of 5 min, fly behavior was recorded using a web camera (logicool quickcam im, logitech, fremont, ca). images of individual flies were captured at 15 frames/s for 10 min and analyzed using pysolo () to track fly locomotion and compute x, y coordinates during every frame for a total of 9000 frames. the percentage of time a fly spent inside an inner circle (70% diameter of the chamber) […]