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Protocols

qbase+ specifications

Information


Unique identifier OMICS_02320
Name qbase+
Software type Toolkit/Suite
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Unix/Linux
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes

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No version available

Publication for qbase+

qbase+ citations

 (171)
call_split

Expressed repetitive elements are broadly applicable reference targets for normalization of reverse transcription qPCR data in mice

2018
Sci Rep
PMCID: 5955877
PMID: 29769563
DOI: 10.1038/s41598-018-25389-6
call_split See protocol

[…] Statistics and data analysis was performed as previously described. In short, the geNorm module in qbase+ version 2.6.1 (Biogazelle, http://www.qbaseplus.com) was used to calculate expression stability values for all reference targets (M-value) and to determine the optimal number of reference targe […]

library_books

Selection and Validation of Reference Genes for Quantitative Real Time PCR Normalization Under Ethanol Stress Conditions in Oenococcus oeni SD 2a

2018
Front Microbiol
PMCID: 5946679
PMID: 29780378
DOI: 10.3389/fmicb.2018.00892

[…] geNorm(qbase+)is a statistical algorithm, which is based on the principle that the expression ratio of two reference genes should be constant in all samples, regardless of the experimental condition, or samp […]

call_split

Hypoxia Induced Cisplatin Resistance in Non Small Cell Lung Cancer Cells Is Mediated by HIF 1α and Mutant p53 and Can Be Overcome by Induction of Oxidative Stress

2018
PMCID: 5923381
PMID: 29690507
DOI: 10.3390/cancers10040126
call_split See protocol

[…] CR was performed as previously described []. A panel of 8 potential housekeeping genes (HKGs) (SDHA1, HMBS, GAPDH, YWHAZ, PMM1, RPL13A, B2M, and HPRT1) were tested on 8 representative samples and the qbase+ software (Biogazelle, Zwijnaarde, Belgium) was used to determine the most stable HKGs. B2M and HPRT1 were selected as optimal reference targets (geNorm M < 0.5 and geNorm V < 0.15). P21, BAX, P […]

library_books

Molecular Preadaptation to Antimony Resistance in Leishmania donovani on the Indian Subcontinent

2018
PMCID: 5907651
PMID: 29669889
DOI: 10.1128/mSphere.00548-17

[…] instructions. qPCRs were run on a LightCycler 480 (Roche) with a SensiMix SYBR No-ROX kit (Bioline) as the SYBR green source; the primers used are displayed in . Expression levels were assessed with qBase+ (Biogazelle) using for normalization two genes previously shown to be very stable: SAT (LdBPK_340035000) () and LdBPK_240021200 (). The expression of both normalization targets was confirmed by […]

library_books

Refined protocols of tamoxifen injection for inducible DNA recombination in mouse astroglia

2018
Sci Rep
PMCID: 5897555
PMID: 29651133
DOI: 10.1038/s41598-018-24085-9

[…] s after five consecutive TAM injections. Reactions were carried out in triplicates with neuregulin 1 type III (NrgIII) and β-actin (bact) as endogenous gene controls. Data normalization and analysis (qbase+, Biogazelle, Gent, Belgium) was based on the ΔΔCT-method. Primers for qRT-PCR were as follows (in 5′ to 3′ direction): NrgIII-forward: GTG TGC GGA GAA GGA GAA AAC T; NrgIII-reverse: AGG CAC AGA […]

call_split

Octreotide Does Not Inhibit Proliferation in Five Neuroendocrine Tumor Cell Lines

2018
PMCID: 5897986
PMID: 29681888
DOI: 10.3389/fendo.2018.00146
call_split See protocol

[…] ces are indicated in Table S1 in Supplementary Material. Plotted values were normalized to ALG9 and HPRT1 using the ΔΔCt method (). Reference genes were validated and chosen using geNorm algorithm by qbase+ software (Biogazelle, Ghent, Belgium). […]


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qbase+ institution(s)
Center for Medical Genetics, Ghent University Hospital, De Pintelaan, Ghent, Belgium

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