qbase+ specifications

Information


Unique identifier OMICS_02320
Name qbase+
Software type Toolkit/Suite
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Unix/Linux
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes

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Publication for qbase+

qbase+ in publications

 (3)
PMCID: 5840212
PMID: 29552555
DOI: 10.3389/fchem.2018.00034

[…] to the manufacturer's instructions. the average values of three biological samples were used to calculate the relative fold expression. the relative fold in expression levels were calculated using qbase+software (hellemans et al., ) by normalizing with actin reference gene (condori et al., )., known stress responsive genes (supplementary table ) expression was measured in homozygous peanut […]

PMCID: 5406548
PMID: 28448946
DOI: 10.1016/j.redox.2017.04.024

[…] center, amsterdam, the netherlands) as described by ramaker et al. . prior to the actual study the most suitable normalization strategy was evaluated by genorm analysis (part of biogazelle's qbase+2.6 software) with 8 representative samples and 8 possible reference genes. based on that target expression levels were normalized to a reference gene index from the expression of actin-beta […]

PMCID: 4169610
PMID: 25317274
DOI: 10.3402/jev.v3.24858

[…] for 30 seconds, then 95°c for 5 seconds and 60°c for 15 seconds (for 45 cycles). primepcr assays that were used for qpcr are listed in supplementary table 1. data were processed and normalized using qbase+2.6 software (www.biogazelle.com). assays with too low an expression level (i.e. missing values in multiple samples) were excluded. three reference genes (cyb5a, rcl1 and syngr2) were selected […]


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qbase+ institution(s)
Center for Medical Genetics, Ghent University Hospital, De Pintelaan, Ghent, Belgium

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