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QFSM specifications


Unique identifier OMICS_26741
Alternative name Quantitative Fluorescent Speckle Microscopy
Software type Application/Script
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS
Programming languages MATLAB
Computer skills Medium
Version 1.0.1
Stability Stable
Maintained Yes




No version available


  • person_outline Gaudenz Danuser

Publication for Quantitative Fluorescent Speckle Microscopy

QFSM citations


Nanoscale Dynamism of Actin Enables Secretory Function in Cytolytic Cells

Curr Biol
PMCID: 5835143
PMID: 29398219
DOI: 10.1016/j.cub.2017.12.044

[…] a generally higher turnover of actin within the IS. This hypothesis could be further explored by analyzing local actin turnover using fluorescence recovery after photobleaching (FRAP) methodology or quantitative fluorescent speckle microscopy (qFSM) []. The most striking difference between NK and T cells is in the kinetics of synapse formation leading to a later range of times for degranulation i […]


Competition of two distinct actin networks for actin defines a bistable switch for cell polarization

Nat Cell Biol
PMCID: 4628555
PMID: 26414403
DOI: 10.1038/ncb3246

[…] obtained with these two methods were highly consistent. Centripetal flow of actin bundles was analyzed using the Kymograph function in MetaMorph 7.7 software. Manual kymographic analysis or automated quantitative fluorescent speckle microscopy (qFSM) tools were applied to analyze the retrograde actin flow in lamellipodial regions of cells expressing low levels of Lifeact. The actin polymerization […]


Growth cone specific functions of XMAP215 in restricting microtubule dynamics and promoting axonal outgrowth

PMCID: 3907036
PMID: 24289819
DOI: 10.1186/1749-8104-8-22

[…] Flow vectors were calculated using QFSM software []. To visualize F-actin flow, neuronal cultures were incubated in 3 nM kabarimide C conjugated to tetramethylrhodamine (TMR-KabC; gift from Tim Gomez) for three minutes, then washed wit […]


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QFSM institution(s)
Department of Cell Biology, Harvard Medical School, Boston, MA, USA

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