qPCR steps

qPCR workflow

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qPCR workflow schema
Quantitative real-time polymerase chain reaction (qPCR) is widely used for the detection of specific nucleic acids, measurement of RNA transcript abundance and validation of high-throughput experimental results. qPCR is often performed in standard…

qPCR software tools

Most Recent Tools

MIPE
Desktop

MIPE MIcrobiota Prior knowledge Explorer

Combines automated small subunit (SSU) primer evaluation with rRNA gene…

Combines automated small subunit (SSU) primer evaluation with rRNA gene extraction and classification for the analysis of shotgun metagenomic and metatranscriptomic datasets. MIPE is a metagenome…

G T A T C G C T A
UBiT2
Web
Desktop

UBiT2 User-friendly BioInformatics Tools

Provides installation-free, offline alignment, analysis, and visualization of…

Provides installation-free, offline alignment, analysis, and visualization of RNA-sequencing as well as qPCR data. UBiT2 can perform gene set enrichment testing by using the non-parametric minimal…

FlexGrePPS
Desktop

FlexGrePPS Flexible Greedy Peptide Pool Search

Provides a solution for proteomic compression. FlexGrePPS is an algorithm for…

Provides a solution for proteomic compression. FlexGrePPS is an algorithm for the construction of near optimal sets of degenerate peptide pools that lend themselves to rapid and economical screening…

SNPman
Desktop

SNPman

Calls the genotypes of single nucleotide polymorphisms (SNP) from TaqMan…

Calls the genotypes of single nucleotide polymorphisms (SNP) from TaqMan allelic discrimination assays. SNPman utilizes the fluorescence data collected over the whole polymerase chain reaction (PCR)…

Most Popular Tools

RefFinder
Web

RefFinder

A user-friendly web-based comprehensive tool developed for evaluating and…

A user-friendly web-based comprehensive tool developed for evaluating and screening reference genes from extensive experimental datasets.

GETPrime
Web

GETPrime

A primer database supported by a novel platform that uniquely combines and…

A primer database supported by a novel platform that uniquely combines and automates several features critical for optimal qPCR primer design.

Curators for qPCR

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