qPCR steps

qPCR workflow

Click the icons of the schema to view tools

qPCR workflow schema
Quantitative real-time polymerase chain reaction (qPCR) is widely used for the detection of specific nucleic acids, measurement of RNA transcript abundance and validation of high-throughput experimental results. qPCR is often performed in standard…

qPCR software tools

Most Recent Tools

PriMux
Desktop

PriMux

Employs suffix array methods for efficient calculations on oligos 10-100 nt in…

Employs suffix array methods for efficient calculations on oligos 10-100 nt in length. PriMux is a software package for selecting multiplex compatible, degenerate primers and probes to detect diverse…

MultiPLX
Desktop
Web

MultiPLX

Provides a tool for automatic grouping of polymerase chain reaction (PCR)…

Provides a tool for automatic grouping of polymerase chain reaction (PCR) primers for multiplexed PCR. MultiPLX performs grouping by calculating many important interaction levels between the…

G T A T C G C T A
msatcommander
Desktop

msatcommander

Is designed to search for microsatellite arrays, design primers, and tag…

Is designed to search for microsatellite arrays, design primers, and tag primers using an automated routine. msatcommander accepts as input DNA sequence data in single-sequence or concatenated,…

eprimer32
Desktop

eprimer32

Pick Polymerase Chain Reaction (PCR) primers and hybridization oligos.…

Pick Polymerase Chain Reaction (PCR) primers and hybridization oligos. eprimer32 is an interface to the 'primer3' program (2.x series) from the Whitehead Institute. The Whitehead program…

Most Popular Tools

RefFinder
Web

RefFinder

A user-friendly web-based comprehensive tool developed for evaluating and…

A user-friendly web-based comprehensive tool developed for evaluating and screening reference genes from extensive experimental datasets.

GETPrime
Web

GETPrime

A primer database supported by a novel platform that uniquely combines and…

A primer database supported by a novel platform that uniquely combines and automates several features critical for optimal qPCR primer design.

Curators for qPCR

Related Websites
Information

By using OMICtools you acknowledge that you have read and accepted the terms of the end user license agreement.