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Quantitative real-time polymerase chain reaction software tools

Quantitative real-time polymerase chain reaction (qPCR) is widely used for the detection of specific nucleic acids, measurement of RNA transcript abundance and validation of high-throughput experimental results. qPCR is often performed in standard 96-well plates, and newer…

qPCR steps

qPCR workflow

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qPCR workflow schema

Experts in qPCR

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Most recent bioinformatics tools

G T A T C G C T A
GrandPrix
Desktop

GrandPrix

Allows users to predict a number of latent dimensions. GrandPrix assists users…

Allows users to predict a number of latent dimensions. GrandPrix assists users in analyzing the single-cell data. This permits researchers to examine the temporal dynamics of complex biological…

G T A T C G C T A
SPADS
Web

SPADS Specific Primers and Amplicon Design Software

Automates the selection of polymerase chain reaction (PCR) amplicons in the…

Automates the selection of polymerase chain reaction (PCR) amplicons in the least conserved regions within a group of genes. SPADS works on the genomic sequence and takes into account the intron-exon…

NTMG
Web

NTMG N-terminal Truncated Mutants Generator for cDNA

Performs automatic multiplex polymerase chain reaction (PCR) assays design.…

Performs automatic multiplex polymerase chain reaction (PCR) assays design. NTMG was developed to generate various N-terminal truncated mutants. It (i) finds ATG-like codons that are suitable to act…

OHM
Web

OHM OligoHeatMap

Estimates Tm for a set of oligomers and a set of aligned sequences. OHM is a…

Estimates Tm for a set of oligomers and a set of aligned sequences. OHM is a web application that extracts and compares the areas of hybridization. The main window contains four frames: (i) to load…

Most popular analytical software

RefFinder
Web

RefFinder

Serves for screening and evaluating reference genes from extensive experimental…

Serves for screening and evaluating reference genes from extensive experimental datasets. RefFinder uses computational programs (such as geNorm or Normfinder) to rank and compare candidate reference…

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