qPCR steps

qPCR workflow

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qPCR workflow schema
Quantitative real-time polymerase chain reaction (qPCR) is widely used for the detection of specific nucleic acids, measurement of RNA transcript abundance and validation of high-throughput experimental results. qPCR is often performed in standard…

qPCR software tools

Most Recent Tools

Web app
Desktop app
Multiplex Primer… Multiplex Primer Design

MPD Multiplex Primer Design

Automates the design of multiplex PCR primers for next-generation sequencing.…

Automates the design of multiplex PCR primers for next-generation sequencing. The core of MPD is implemented in C for speed and uses a hashed genome to ensure primer uniqueness, avoids placing…

Web app
Chainy Chainy

Chainy

Provides systematic pipelines and steady criteria to process real-time PCR…

Provides systematic pipelines and steady criteria to process real-time PCR data. Chainy includes the calculation of efficiencies from raw data by kinetic methods, evaluation of the suitability of…

Desktop app
qpcrNorm qpcrNorm

qpcrNorm

Contains functions to perform normalization of high-throughput qPCR data.…

Contains functions to perform normalization of high-throughput qPCR data. qpcrNorm calculates the coefficient of variation for each gene in the qPCR experiment, and returns the average coefficient of…

Desktop app
Tool for PCR… Tool for PCR Signature Identification

TOPSI Tool for PCR Signature Identification

A high-performance computing pipeline for the design of PCR-based pathogen…

A high-performance computing pipeline for the design of PCR-based pathogen diagnostic assays. The TOPSI pipeline efficiently designs PCR signatures common to multiple bacterial genomes by obtaining…

Most Popular Tools

Web app
RefFinder Deprecated RefFinder

RefFinder

A user-friendly web-based comprehensive tool developed for evaluating and…

A user-friendly web-based comprehensive tool developed for evaluating and screening reference genes from extensive experimental datasets.

Web app
GETPrime GETPrime

GETPrime

A primer database supported by a novel platform that uniquely combines and…

A primer database supported by a novel platform that uniquely combines and automates several features critical for optimal qPCR primer design.

Curators for qPCR

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