Quantitative real-time polymerase chain reaction software tools

Quantitative real-time polymerase chain reaction (qPCR) is widely used for the detection of specific nucleic acids, measurement of RNA transcript abundance and validation of high-throughput experimental results. qPCR is often performed in standard 96-well plates, and newer…

qPCR steps

qPCR workflow

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qPCR workflow schema

Most recent bioinformatics tools

ICG
Dataset

ICG Internal Control Genes

Provides access to information about internal control genes for Real-time…

Provides access to information about internal control genes for Real-time quantitative (RT-qPCR) normalization. ICG is a wiki-based, publicly editable and open-content resource for community curation…

OligoGenome
Dataset

OligoGenome Stanford Human Oligo Genome Project

Provides oligonucleotide capture sequences that span the human genome.…

Provides oligonucleotide capture sequences that span the human genome. OligoGenome offers functions to browse, filter and download capture oligonucleotide sequences based upon user queried genomic…

RefPrimeCouch
Dataset

RefPrimeCouch A Reference Gene Primer CouchApp

Allows user to find information about gene. RefPrimeCouch assists user in…

Allows user to find information about gene. RefPrimeCouch assists user in development of quantitative real-time polymerase chain reaction (qPCR) standardization project. This resource includes…

HIVoligoDB
Dataset

HIVoligoDB The HIV oligonucleotide database

Provides a set of oligonucleotides and genomic regions that can be used to…

Provides a set of oligonucleotides and genomic regions that can be used to improve the efficiency of Human Immunodeficiency Virus (HIV) epidemiological studies and nucleic acid-based assays.…

Most popular analytical software

RefFinder
Web

RefFinder

Serves for screening and evaluating reference genes from extensive experimental…

Serves for screening and evaluating reference genes from extensive experimental datasets. RefFinder uses computational programs (such as geNorm or Normfinder) to rank and compare candidate reference…

Curators for qPCR

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