QUALIFIER

[…] ature 400 °C. In positive ion MRM mode and with collision energy of 29 V, two product ions were monitored after CID of the S1P precursors. M/z 60 was used as a ‘quantifier’ and m/z 113 was used as a ‘qualifier’, as reported previously. These ions were present after fragmentation of all the derivatised LCB-P species. Quantification was performed according to the internal standard method, comparing […]

[…] are filtered out. MRM was developed to detect and quantify both the non-adenylylated and adenylylated forms of the peptide using the transitions: 606.3 > 954.5, 770.8 > 136.1 respectively, additional qualifier ions 606.3 > 244.2, 606.3 > 791.4, for the native protein and, 770.8 > 244.2, 770.8 > 1291.7 for the adenylylated form were used. For further validation that the GAEYSVDFVPK peptide was aden […]

[…] , accessed by entering a gene or feature name, displays a simple table containing all existing phenotypes curated for that gene, and uses scroll boxes containing the mutant type, experiment type, and qualifier hierarchies for entering new phenotype annotations. The YPO and strain background-controlled vocabularies are accessed via pop-up windows, and there is a link to open the ChEBI website in a […]