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QuantStudio 3D AnalysisSuite Cloud Software specifications

Information


Unique identifier OMICS_03946
Name QuantStudio 3D AnalysisSuite Cloud Software
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Biological technology Life Technologies
Operating system Unix/Linux
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes

Versioning


No version available

QuantStudio 3D AnalysisSuite Cloud Software citations

 (11)
library_books

Digital PCR for gene expression: impact from inherent tissue RNA degradation

2017
Sci Rep
PMCID: 5722939
PMID: 29222437
DOI: 10.1038/s41598-017-17619-0

[…] to running duplex reactions, assays were validated to ensure the duplex readings were consistent with respective single-plex assay readings over a range of input cDNA concentrations (data not shown). Quantstudio 3D AnalysisSuite Cloud Software (Applied Biosystems, version 3.1.2-PRC-build-03) with Poisson Plus quantification algorithm (version 4.4.10) and 95% CI was used. Imported eds files were in […]

library_books

Poisson Plus Quantification for Digital PCR Systems

2017
Sci Rep
PMCID: 5575031
PMID: 28851915
DOI: 10.1038/s41598-017-09183-4

[…] 454.44454.4−541v¯¯)×CVvAssuming a mean through-hole fill volume of 755 picoliters:18CVR=1.0002×CVvIt is thus reasonable to assume CV in ROX is representative of the CV in through-hole fill volume. In QuantStudio 3D AnalysisSuite Cloud Software, as ROX readings are available for each chip, the coefficient of variation is estimated on a per chip basis, which is, in turn, used to correct for the leve […]

library_books

Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples

2017
Microbiome
PMCID: 5549297
PMID: 28789678
DOI: 10.1186/s40168-017-0317-z

[…] y 10 min at 96 °C. Cycling conditions were 50 cycles of 2 min at 60 °C, 30 s at 98 °C, and 60 °C for 2 min. After read-out on the QuantStudio 3D Digital PCR instrument, the raw data was imported into QuantStudio 3D AnalysisSuite Cloud Software (version 3.0.2.2) to calculate absolute copy numbers. […]

library_books

Nanofluidic digital PCR for the quantification of Norovirus for water quality assessment

2017
PLoS One
PMCID: 5531372
PMID: 28749991
DOI: 10.1371/journal.pone.0179985

[…] step for 2 min at 60°C.QuantStudio 3D Digital PCR instrument (ThermoScientific, US) was used to count the number of positive wells out of the total number of well per chip and the Applied Biosystems™ QuantStudio™ 3D AnalysisSuite™ Cloud Software were used to analyse and refine the data derived from QuantStudio 3D Digital PCR instrument. Poisson distribution was used to estimate the average number […]

library_books

Cerebrospinal fluid microRNAs are potential biomarkers of temporal lobe epilepsy and status epilepticus

2017
Sci Rep
PMCID: 5468228
PMID: 28607431
DOI: 10.1038/s41598-017-02969-6

[…] ly processed using the QuantStudio 3D instrument to obtain the number of wells with an amplified target (FAM positive) and number of empty wells (FAM negative). Copy numbers were determined using the QuantStudio 3D AnalysisSuite Cloud software. […]

library_books

Detection of newly produced T and B lymphocytes by digital PCR in blood stored dry on nylon flocked swabs

2017
J Transl Med
PMCID: 5381048
PMID: 28381232
DOI: 10.1186/s12967-017-1169-9

[…] (or KRECs) and 200 nM of probe for TRECs FAM-TAMRA (or KRECs VIC-TAMRA), and 0.29 μL of BSA (2 mg/mL).Absolute quantification was determined using Quantstudio® 3D Digital PCR System and analyzed with QuantStudio® 3D AnalysisSuite Cloud Software (Thermo Fisher Scientific).Sensitivity and specificity of dPCR platform were evaluated with the plasmid created for qRT-PCR diluted in the DNA from HeLa ce […]

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QuantStudio 3D AnalysisSuite Cloud Software institution(s)
ThermoFisher Scientific

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