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QuickPALM specifications

Information


Unique identifier OMICS_06524
Name QuickPALM
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux
License GNU General Public License version 2.0
Computer skills Medium
Stability Stable
Maintained Yes

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Documentation


Publication for QuickPALM

QuickPALM citations

 (35)
library_books

Single cardiac ventricular myosins are autonomous motors

2018
Open Biol
PMCID: 5936712
PMID: 29669825
DOI: 10.1098/rsob.170240

[…] using the conversion formula in solis and the images output in tiff format for reading into imagej. we tracked the movement of the qdot labelled actin at super resolution using the imagej plugin quickpalm []. baseline histograms corresponding to thermal/mechanical fluctuations were recorded likewise from qdot labelled actin immobilized on the surface by myosin in the absence of atp. […]

library_books

Temporal reprogramming of calcium signalling via crosstalk of gonadotrophin receptors that associate as functionally asymmetric heteromers

2018
Sci Rep
PMCID: 5797151
PMID: 29396488
DOI: 10.1038/s41598-018-20722-5

[…] using fiji software., fluorescent intensity images of cropped non-overlapping 7 × 7 μm areas, within cell-cell boundaries, from 491- and 561-nm channels were analysed for localized receptors by quickpalm fiji plugin as previously described. data tables containing the x-y coordinates of localized receptors in 491- and 561-nm channels generated., the number of associated receptor protomers […]

library_books

A Method for Quantifying Molecular Interactions Using Stochastic Modelling and Super Resolution Microscopy

2017
Sci Rep
PMCID: 5665986
PMID: 29093506
DOI: 10.1038/s41598-017-14922-8

[…] catalase, and 10% glucose),. movies containing a minimum of 2,000 frames were used to generate reconstructed super-resolved images., sr image reconstruction was performed using the freely available quickpalm plug-in for imagej. we specifically used an fwhm of 4 and a signal-to-noise ratio (snr) of 2–4 to detect localized particles from our raw data. localized molecules were written to rendered […]

library_books

Correlative super resolution fluorescence and electron microscopy using conventional fluorescent proteins in vacuo

2017
J Struct Biol
PMCID: 5531056
PMID: 28576556
DOI: 10.1016/j.jsb.2017.05.013

[…] preliminary super-resolution data acquisition in order to determine which worked better with the data generated by the ilsem-based system, including 3b (), csstorm (), localizer (), octane (), quickpalm (), rainstorm () and rapidstorm (). the patterns of particle localisation obtained were remarkably similar, but with tremendous variation in processing time and flexibility of operation […]

library_books

Plakophilin 2 is required for transcription of genes that control calcium cycling and cardiac rhythm

2017
Nat Commun
PMCID: 5524637
PMID: 28740174
DOI: 10.1038/s41467-017-00127-0

[…] was used to image two colors simultaneously, side-by-side, onto a em-ccd camera (andor ixon+897). two-color movies containing 2000 frames were processed using an imagej macro routine based on the quickpalm plugin. the two-color image was split into its separate channels and each reconstructed at 20 nm using the quickpalm parameters full-width half-maximum 4 and signal/noise ratio 4. […]

library_books

Flat clathrin lattices are dynamic actin controlled hubs for clathrin mediated endocytosis and signalling of specific receptors

2017
Nat Commun
PMCID: 5511353
PMID: 28703125
DOI: 10.1038/ncomms16068

[…] images were convolved with a mean filter of 3 × 3 pixels. for , images were analysed also using the default parameters of the analysis software provided by leica las-af and the open source software quickpalm and rapidstorm., sr image quantification: plaques and pits were segmented and analysed with the particle analyser routine from fiji (imagej) with manually adjusted threshold. a 3 μm-wide […]


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