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Citations per year

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Protocols

Quimp specifications

Information


Unique identifier OMICS_06580
Name Quimp
Software type Package/Module
Interface Graphical user interface
Restrictions to use Academic or non-commercial use
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java, MATLAB
Computer skills Medium
Version 18.02.01
Stability Stable
Maintained Yes

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Maintainer


  • person_outline Till Bretschneider

Publications for Quimp

Quimp citations

 (18)
library_books

Green monomeric photosensitizing fluorescent protein for photo inducible protein inactivation and cell ablation

2018
BMC Biol
PMCID: 5928576
PMID: 29712573
DOI: 10.1186/s12915-018-0514-7

[…] under a 60× oil immersion objective lens NA 1.4 (Nikon) for 10 s. Images of Venus and mNeptune fluorescence were taken using a 488 nm laser and a 633 nm laser. Image analysis was performed using the QuimP plugin [] in Fiji Software [] to measure the fluorescence intensity in the cytoplasm and plasma membrane of cells. The ratio increase presented in Fig. was calculated as:ΔRatio=IcytoplasmtxImem […]

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STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo

2017
Proc Natl Acad Sci U S A
PMCID: 5754794
PMID: 29203676
DOI: 10.1073/pnas.1713535114
call_split See protocol

[…] eica DFC 450C camera. The cell area and circularity measurements were performed using ImageJ. The total cortical actin (4 µm from the cell edge) normalized to cytosolic actin was quantified using the QuimP (version 17.06.02) plug-in in ImageJ. The number and area of focal adhesions were based on paxillin staining and quantified as described ().For the analyses of cellular protrusions, the four ind […]

library_books

Coupled excitable Ras and F actin activation mediates spontaneous pseudopod formation and directed cell movement

2017
Mol Biol Cell
PMCID: 5385941
PMID: 28148648
DOI: 10.1091/mbc.E16-10-0733

[…] and 3 s–1 (; ). Movies of cells coexpressing RBD-Raf-GFP and LifeAct-RFP from one plasmid were recorded on a confocal microscope at 1.18 s/frame. The extension of a new pseudopod was determined using Quimp3 as described (). This provided the first frame (t0) and starting position at the cell boundary of a new protrusion. A line scan (width 660 nm) was drawn perpendicular to the cell surface at thi […]

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Method to study cell migration under uniaxial compression

2017
Mol Biol Cell
PMCID: 5349787
PMID: 28122819
DOI: 10.1091/mbc.E16-08-0575
call_split See protocol

[…] e projections. Numbers of blebs and pseudopods formed by a cell were counted, and the ratio of blebs and total projections was calculated.The speed of cells was calculated by automated tracking using QUIMP plug-in in ImageJ (). The centroid of cells was tracked at every frame of the movie to calculate the total distance covered by the cell. It was then divided by total time to calculate the speed […]

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Kindlin 1 Regulates Keratinocyte Electrotaxis

2016
PMCID: 5756539
PMID: 27427485
DOI: 10.1016/j.jid.2016.05.129
call_split See protocol

[…] Pseudopod dynamics analysis was conducted with the Quimp3 algorithm following a previous description (). Briefly, the recorded cell migration movie was tracked using ImageJ software (National Institutes of Health) with the pseudopod macro. The movemen […]

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A plasma membrane template for macropinocytic cups

2016
eLife
PMCID: 5154761
PMID: 27960076
DOI: 10.7554/eLife.20085.032
call_split See protocol

[…] a calculated point spread function. The images presented are maximum intensity projections of the deconvolved dataset.Correlation between speed and membrane fluorescence intensity was analysed using Quimp11 (www.warwick.ac.uk/quimp). Identification of membrane pixels and measuring their fluorescence intensity was done using a custom-written MATLAB (The MathWorks) script ( and ).Image sets that we […]


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Quimp institution(s)
Department of Computer Science & Zeeman Institute, University of Warwick, Coventry, UK
Quimp funding source(s)
Supported by BBSRC (BB/M01150X/1).

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