An open-access and curated reference barcoding database for diatoms, developed in the framework of R-Syst, the network of systematic supported by INRA (French National Institute for Agricultural Research). R-Syst::diatom links DNA-barcodes to their taxonomical identifications, and is dedicated to identify barcodes from natural samples. The data come from two sources, a culture collection of freshwater algae maintained in INRA in which new strains are regularly deposited and barcoded and from the NCBI (National Center for Biotechnology Information) nucleotide database. Two kinds of barcodes were chosen to support the database: 18S (18S ribosomal RNA) and rbcL (Ribulose-1,5-bisphosphate carboxylase/oxygenase), because of their efficiency. Data are curated using innovative (Declic) and classical bioinformatic tools (Blast, classical phylogenies) and up-to-date taxonomy (Catalogues and peer reviewed papers). In addition to these information, morphological features (e.g. biovolumes, chloroplasts...), life-forms (mobility, colony-type) or ecological features (taxa preferenda to pollution) are indicated in R-Syst::diatom.
INRA—UMR Carrtel, Thonon Les Bains, France; UMR CARRTEL, University of Savoie, Le Bourget Du Lac, France; INRA, UMR BioGeCo, Cestas, France; University of Bordeaux 1, UMR BioGeCo, Talence, France; ASCONIT Consultants, Naturopôle, Toulouges, France; Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, Uppsala, Sweden