rapidSTORM specifications


Unique identifier OMICS_06525
Name rapidSTORM
Software type Package/Module
Interface Command line interface, Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes


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Publication for rapidSTORM

rapidSTORM in publications

PMCID: 5915489
PMID: 29720981
DOI: 10.3389/fimmu.2018.00815

[…] of cd3 molecules on the basal membrane of the cells. for each dstorm measurement 15,000 images with an exposure time of 20 ms and an irradiation intensity of ~5 kw/cm2 were recorded., the software rapidstorm 3.2 was used to localize single-molecule events in all recorded images, to generate a localization file with all localizations, and to reconstruct the dstorm image. localizations […]

PMCID: 5884788
PMID: 29618762
DOI: 10.1038/s41598-018-24052-4

[…] control software. once imaging was complete, the beamshaper was either added to, or removed from, the optical path, and the imaging process repeated., fluorophore localization was performed using rapidstorm; the super-resolved image was generated using rapidstorm itself, while further analysis of the fluorophore density was performed by importing the fluorophore locations into matlab 2017a […]

PMCID: 5834503
PMID: 29500346
DOI: 10.1038/s41467-018-03191-2

[…] size of 129 nm. the camera, microscope and aotf were controlled by µmanager software on a pc workstation. single-molecule localizations were extracted from the movies with the open-source software rapidstorm 3.2. drift correction was performed by custom written python 2.7 algorithms that extract and correct for fluorescent bead tracks. nena as described in ref. was done with the open-source […]

PMCID: 5818535
PMID: 29459792
DOI: 10.1038/s41467-018-03111-4

[…] imaging was performed in highly inclined (hilo) illumination mode. overall, 16,000 image frames with exposure times between 7.18 and 20 ms were recorded and subsequently reconstructed using either rapidstorm 3.3 or the open-source rainstorm software developed in-house written in matlab (the mathwork inc., natick, usa). image overlays were assembled in fiji, experiments described were repeated […]

PMCID: 5732201
PMID: 29247204
DOI: 10.1038/s41598-017-18071-w

[…] an electron-multiplying ccd camera (ixon ultra du-897; andor). the sample was homogenously illuminated with an irradiation intensity of ~ 7 kw/cm2 and super-resolved images were reconstructed with rapidstorm 3.3.1, from 30,000 frames with an exposure time of 20 ms. lsm was performed with a lsm700 (zeiss, germany) equipped with a plan-apochromat 63 × 1.4 oil-immersion objective […]

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