An algorithm for automated three-dimensional shape analysis from laser scanning microscopy images. The Rayburst algorithm provides a primitive for the development of higher level algorithms that solve specific shape analysis problems. Examples are provided of applications to 3D neuronal morphometry: (i) estimation of diameters in tubular neuronal dendritic branching structures, and (ii) measurement of volumes and surface areas for dendritic spines and spatially complex histopathologic structures.
Fishberg Department of Neuroscience, Mount Sinai School of Medicine, New York, NY, USA; Center for Biomathematical Sciences, Mount Sinai School of Medicine, New York, NY, USA; Computational Neurobiology and Imaging Center, Mount Sinai School of Medicine, New York, NY, USA
Rayburst sampling funding source(s)
This work was supported by NIH grants from NIMH, NIDCD, NIA and NCRR.