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RBSDesigner specifications


Unique identifier OMICS_08702
Name RBSDesigner
Interface Web user interface
Restrictions to use None
Programming languages Python
Computer skills Basic
Version 1.0.78
Stability Stable
Maintained Yes


  • person_outline RBSDesigner <>

Publication for RBSDesigner

RBSDesigner citations


Novel Functions and Regulation of Cryptic Cellobiose Operons in Escherichia coli

PMCID: 4488073
PMID: 26121029
DOI: 10.1371/journal.pone.0131928

[…] regions of chbb, chba, chbc, chbf, ascf and ascb genes and the promoter regions of chb and asc operon. oss strains were transformed with the plasmid psim5. the rbs location was predicted using the rbsdesigner program []. multiplex genome engineering was performed as described elsewhere but with a simple modification []. seven oligos were mixed at a concentration of 0.3 μm per oligo. following […]


Synthetic Biology Toolbox for Controlling Gene Expression in the Cyanobacterium Synechococcus sp. strain PCC 7002

PMCID: 4431953
PMID: 25216157
DOI: 10.1021/sb500260k

[…] of the model from r2 = 0.3760 to r2 = 0.7443 (figure a and b). the rbs library sequences were also back calculated with two alternative translation rate prediction programs: the utr designer and the rbsdesigner. in this case, the correlation of the experimental data to the predicted expression level provided by the utr designer (figure c) was similar to the rbs calculator prediction; however, […]


Discovery of a super strong promoter enables efficient production of heterologous proteins in cyanobacteria

PMCID: 3968457
PMID: 24675756
DOI: 10.1038/srep04500

[…] the c-phycocyanin beta subunit, while a human cuzn superoxide dismutase gene was expressed in synechococcus sp. 7002 using the promoter and terminator from the rbc operon, which encodes rubisco., rbsdesigner predicted that the rbs in the cpcb promoter is approximately 10-times more efficient than that in the rbc promoter in terms of the predicted translation efficiency. therefore, we chose […]


Switchable Gene Expression in Escherichia coli Using a Miniaturized Photobioreactor

PMCID: 3547951
PMID: 23349683
DOI: 10.1371/journal.pone.0052382

[…] plasmids, respectively. plasmid pjm1 and pjm2 are different from each other in their rbs sequence, with strong rbs in the former and weak rbs in the later. rbs sequences were designed using the rbsdesigner software (http://rbs.kaist.ac.kr/index.html) that determines the strength of a particular rbs (translational efficiency) using a relative scale from 0 to 1 . the translational efficiency […]

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RBSDesigner institution(s)
Department of Bio and Brain Engineering, KAIST, Gwahangno, Yuseong-gu, Daejeon, Korea

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