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Genome-guided transcriptome assembly software tools | RNA sequencing data analysis

Genome-guided transcriptome assembly software tools | RNA sequencing data analysis A reference-based or genome-guided transcriptome assembly algorithm uses alignments of reads to the genome that are produced by a specialized spliced-alignment tool, such as TopHat2 or GSNAP, to identify clusters of reads that represent potential transcripts. It then builds transcript assemblies from these alignments. If paired-end reads are available, they improve the ability of the assembler to link together exons belonging to the same transcript.
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