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Reference genes software tools | qPCR analysis

To normalize RT-qPCR measurements between samples, most laboratories use endogenous reference genes as internal controls. Several programs help you to choose the best combination of reference genes for your own experiment.

RefFinder
Web

RefFinder

Serves for screening and evaluating reference genes from extensive experimental…

Serves for screening and evaluating reference genes from extensive experimental datasets. RefFinder uses computational programs (such as geNorm or Normfinder) to rank and compare candidate reference…

NormFinder
Desktop

NormFinder

It ranks the set of candidate normalization genes according to their expression…

It ranks the set of candidate normalization genes according to their expression stability in a given sample set and given experimental design.

BestKeeper
Desktop

BestKeeper

Determines the best suited standards, out of ten candidates, and combines them…

Determines the best suited standards, out of ten candidates, and combines them into an index.

NormqPCR
Desktop

NormqPCR

Provides tools for uploading RT-qPCR data into R, looks for the optimal…

Provides tools for uploading RT-qPCR data into R, looks for the optimal reference genes, and normalises the data using the ΔΔCq method. NormqPCR allows the user to read RT-qPCR data into R, to deal…

RefGenes
Web

RefGenes

An online app from Genevestigator that allows users to search for genes that…

An online app from Genevestigator that allows users to search for genes that are most stable across a chosen set of samples based on microarray data. This set of samples can be chosen according to…

ICG
Dataset

ICG Internal Control Genes

Provides access to information about internal control genes for Real-time…

Provides access to information about internal control genes for Real-time quantitative (RT-qPCR) normalization. ICG is a wiki-based, publicly editable and open-content resource for community curation…

geNorm
Desktop

geNorm

An algorithm to determine the most stable reference (housekeeping) genes from a…

An algorithm to determine the most stable reference (housekeeping) genes from a set of tested candidate reference genes in a given sample panel.

OLIVER
Desktop

OLIVER OLIgonucleotide Variable Expression Ranker

A software tool for identifying reference genes from large datasets. This tool…

A software tool for identifying reference genes from large datasets. This tool compiles eight different methods and had been incorporated as part of Bactome project. OLIVER is dependent on Collection…

RefPrimeCouch
Dataset

RefPrimeCouch A Reference Gene Primer CouchApp

Allows user to find information about gene. RefPrimeCouch assists user in…

Allows user to find information about gene. RefPrimeCouch assists user in development of quantitative real-time polymerase chain reaction (qPCR) standardization project. This resource includes…

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