RELION specifications


Unique identifier OMICS_15597
Alternative name REgularised LIkelihood OptimisatioN
Software type Application/Script
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Programming languages C++
License GNU General Public License version 2.0
Computer skills Medium
Version 2.0
Stability Stable
Maintained Yes



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  • person_outline Sjors Scheres <>

RELION article

RELION citations

PMCID: 5842316

[…] of each micrograph were estimated with ctffind4 (rohou and grigorieff, 2015). in a first step, ∼20,000 particles were picked with the semi-automated swarm method using eman2 (tang et al., 2007). relion (scheres, 2012) was used for the image processing workflow unless stated otherwise. reference-free 2d classes were generated, five of which were used for template-based auto-picking […]

PMCID: 5834697

[…] å in motioncor2 (52). contrast transfer function (ctf) estimates were performed using gctf (53), followed by particle picking and extraction of a total of 469,224 particles from 2,479 micrographs in relion 2.0 (54–56). after several rounds of 2d classification, 352,880 particles in all usable classes were used for 3d classification using a low-pass-filtered map of the cross-linked apo structure […]

PMCID: 5700161

[…] were picked using swarm from eman243 followed by manual removal of false positives and inclusion of false negatives. ctf calculations were done with gctf44 and particle coordinates were imported to relion 2.045, where all next steps were performed. an initial group of 505,880 raw particles were extracted using a 256 × 256 pixel box size and 2d classification was performed. particles belonging […]

PMCID: 5606707

[…] movie s3. similar to the structures of wild-type and untreated complexes, particle orientations were not limited, and the map was refined to 15.6 å according to the 0.5 fsc criteria determined in relion and rmeasure (fig. s3). the brca15382insc-bard1 structure produced under oxidative stress conditions was more compact and had extra density adjacent to the ring domain (fig. 3d)., to better […]

PMCID: 5606707

[…] in movie s1. the particles did not show limited orientations in their angular distribution, and the structure was refined to 14.5 å according to the 0.5 fourier shell correlation (fsc) criteria in relion (fig. s1). the resolution of the map was verified using the rmeasure application (26). the calculated molecular volume of the density map accommodates one brca1-bard1 dimer., […]

RELION institution(s)
MRC Laboratory of Molecular Biology, Cambridge, UK
RELION funding source(s)
This work was funded by the UK Medical Research Council through grant MC_UP_A025_1013.

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