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RELION specifications


Unique identifier OMICS_15597
Alternative name REgularised LIkelihood OptimisatioN
Software type Application/Script
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Programming languages C++
License GNU General Public License version 2.0
Computer skills Medium
Version 3
Stability Stable
Maintained Yes




No version available


  • person_outline Sjors Scheres
  • person_outline Erik Lindahl

Additional information

Publications for REgularised LIkelihood OptimisatioN

RELION citations


Structure of a cleavage independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer

Nat Commun
PMCID: 5955915
PMID: 29769533
DOI: 10.1038/s41467-018-04272-y

[…] ee 2D classification were carried out using MSA/MRA with a binning factor of 2, to remove amorphous particles. After 2D classification, the 2× binned particles were subject to 3D classification using RELION, starting with an initial reference model of an unliganded trimer filtered to 60 Å resolution without imposing symmetry, and requesting six classes. Two stoichiometric classes were identified i […]


Structural and immunologic correlates of chemically stabilized HIV 1 envelope glycoproteins

PLoS Pathog
PMCID: 5944921
PMID: 29746590
DOI: 10.1371/journal.ppat.1006986
call_split See protocol

[…] d to automated difference-of-Gaussian particle selection []. The resulting set of molecular projection-image candidates were binned by a factor of 4 and subjected to reference-free class averaging in Relion 1.4b1 []. Projection images belonging to structural classes were selected for angular refinement and reconstruction using a low-pass filtered unliganded HIV-1 Env trimer density map as a refere […]


Structural basis for cofilin binding and actin filament disassembly

Nat Commun
PMCID: 5945598
PMID: 29749375
DOI: 10.1038/s41467-018-04290-w
call_split See protocol

[…] A total of 1,111 images were collected in two microscope sessions (414 and 697 micrographs, respectively). Image processing was performed with RELION 2.0 software,. After motion correction with MotionCorr and CTF estimation with Gctf, 1,093 images were selected for further image processing. Filaments were manually picked with e2helixboxer, a […]


Cryo EM of nucleosome core particle interactions in trans

Sci Rep
PMCID: 5935684
PMID: 29728587
DOI: 10.1038/s41598-018-25429-1
call_split See protocol

[…] rticles were then used for semi-automatic and automatic particle picking in XMIPP. The contrast transfer function parameters were determined by CTFFIND4. The 2D class averages were generated with the Relion software package. Bad class averages were removed from further data analysis. The 3D refinements and classifications were subsequently done in Relion. All final refinements were done in Relion […]


Structure and mechanism of the two component α helical pore forming toxin YaxAB

Nat Commun
PMCID: 5935710
PMID: 29728606
DOI: 10.1038/s41467-018-04139-2

[…] The image processing workflow was performed with RELION 2.1. Image stacks were motion corrected with the program MotionCor2, after which the contrast transfer function values were estimated and corrected using Kai Zhang’s Gctf. 1009 particles were m […]


Ribosome protection by antibiotic resistance ATP binding cassette protein

Proc Natl Acad Sci U S A
PMCID: 5960329
PMID: 29712846
DOI: 10.1073/pnas.1803313115

[…] mo Fisher Scientific) operated at 300 kV. Data were acquired automatically in movie mode as sets of 20 frames at a nominal magnification of 75,000×. Particles picking and data processing were done in Relion 2.0. A total of 310,270 particles were used for reference-free 2D classification, and nonribosome particles were removed. The remaining 186,654 particles were used for 3D classification using e […]


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RELION institution(s)
MRC Laboratory of Molecular Biology, Cambridge, UK
RELION funding source(s)
Supported by the UK Medical Research Council (MC UP A025 1013); the Swiss National Science Foundation (SNF: P2BSP2 168735); the Swedish Research Council (2017-04641) and the Knut and Alice Wallenberg Foundation.

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