RestrictionMapper protocols

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RestrictionMapper specifications


Unique identifier OMICS_17688
Name RestrictionMapper
Interface Web user interface
Restrictions to use None
Programming languages Perl
Computer skills Basic
Stability Stable
Maintained Yes

RestrictionMapper in pipeline

PMCID: 4815569
PMID: 25940119
DOI: 10.5830/CVJA-2015-019

[…] using the clustalw v1.4 programme and analysed for mismatches to the reference sequence., the effect of sequence variants on restriction enzyme recognition sites was determined using,, and nebcutter, restriction enzyme analysis was then used to confirm genotypes, where possible. digestion […]

RestrictionMapper in publications

PMCID: 5594730
PMID: 28877567
DOI: 10.3347/kjp.2017.55.4.367

[…] lithuania). at first, this enzyme was tested against sequences of reference strains (fasta format genes downloaded from genbank) and searched for restriction sites using restriction mapper 3 ( theoretically, obtained fragments from digestion with haeiii would be expected to create different patterns as follows: l. major (351, 307, 246, 152, 99, 47, 41, 40, 34, and 2 […]

PMCID: 5634817
PMID: 29021970
DOI: 10.1016/j.bdq.2017.08.001

[…] and enzymatic digestion has been suggested to eliminate this effect. prior to ddpcr, genomic dna was digested with a restriction enzyme. sequence of interest was checked for various enzymes using restrictionmapper ( and it was found that hindiii did not digest the sequences of interest and therefore all samples were digested with hindiii prior to pcr. […]

PMCID: 5501944
PMID: 28688454
DOI: 10.1186/s12917-017-1137-x

[…] under uv illumination using the ec3 chemi hr 410 imaging system (ultra-violet products ltd., uk)., restriction enzymes (bamhi, nhei and ecori) were selected for the rflp analysis using version 3 of restrictionmapper (, the cleavage reaction was performed in a final volume of 16 μl containing 5 μl of the amplified product, 1 μl of the adequate restriction […]

PMCID: 5477317
PMID: 28522702
DOI: 10.1128/genomeA.00315-17

[…] 96% identity), which infects e. coli b strains with an incomplete core lipopolysaccharide (). the restriction digestion analysis in silico using restriction mapper version 3 tool ( showed that the alf5 genome sequence contains no recognition sites for many restriction enzymes, including bamhi, xhoi, saci, psti, and even mboi, which recognizes gatc, […]

PMCID: 5450958
PMID: 28506038
DOI: 10.3347/kjp.2017.55.2.159

[…] nos. ku893351 to ku893860) amplified from korean p. vivax isolates [], we surveyed the restriction enzyme sites in each subtype of msp-1 and msp-3α using the restriction mapper program ( the restriction enzymes with digestion sites that were conserved within a subtype in a given sequence were selected. the selected restriction enzymes and their predicted […]

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