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Reverse phase protein array (RPPA) technology, first described in 2001, allows simultaneous measurement of numerous glycosylated, phosphorylated, cleaved, or total cellular proteins from complex mixtures in many samples at once. RPPA is considerably faster than conventional techniques such as mass spectrometry, western blotting, 2-D PAGE.
(Paweletz et al., 2001) Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front. Oncogene.