Ribosome stalling detection software tools | Ribo-seq data analysis
During cellular translation of messenger RNAs by ribosomes, the translation apparatus sometimes pauses or stalls at the elongation and termination steps. With the exception of programmed stalling, which is usually used by cells for regulatory purposes, ribosomes stalled on mRNAs need to be terminated and recycled to maintain adequate translation capacity. Much ribosome stalling originates in aberrant mRNAs that lack a stop codon.
Analyzes large-scale ribosome profiling data and study the contextual regulation of ribosome stalling and its potential functions in protein biogenesis. ROSE formalizes the modeling problem as a classification task, in which the resulting prediction score can be used to measure the probability of a ribosome stalling event. It is able to predict ribosome stalling events in several different datasets generated from other studies.