RNAhybrid

[…] expression in gc tumor tissues (n = 30, r = −0.504, p = 0.0039; ). furthermore, we performed a search for mirnas that have complementary base pairing with gaplinc, using online software program rnahybrid (https://bibiserv.cebitec.uni-bielefeld.de/rnahybrid/). the results demonstrated that mir-378 could form complementary base pairing with gaplinc (). we also observed the expression levels […]

[…] rna and 45.5% (2,018/4,438) had higher expression of the linear rna., given the potential mirna regulatory function of circrnas, we next used the mirna target prediction algorithms miranda [] and rnahybrid [] to predict the mirna targets of the circrnas detected in ten or more samples by at least one of the circrna prediction algorithms (n = 10 circrna candidates). using a list of 2588 […]

[…] bayesian approach was applied for the statistical analysis of the data []., to test for significant associations between genes, mirnas, pathways and go terms, the prediction software targetscan and rnahybrid, together with the differentially expressed genes in the l. vannamei hemocytes transcriptome data (genebank accession numbers: prjna385392) were used. the relatedness or anticorrelation […]

[…] took part in several biological processes in the chicken ovary., based on the data for differentially expressed protein-coding transcripts, mirna, and lncrna transcripts, we used three softwares rnahybrid(v2.1.2) + svm_light(v6.01), miranda(v3.3a) and targetscan(version:7.0) to identify biological targets of each mirna from the protein-coding and lncrna transcripts that showed […]

[…] ubiquitously expressed in the ovarian follicle and important in reproductive performance [], were selected for functional analysis. the secondary structure of mir-424 and mir-503 was predicted by rnahybrid (http://bibiserv.techfak.uni-bielefeld.de/rnahybrid)., bovine ovaries as sources of bgcs were collected from a local slaughterhouse. ovaries were processed to obtain follicular fluid […]

[…] 33 vac-encoded mirnas, only 13 mirna sequences and 22 “seed sequences” of mirna were identical, and 8 novel viral mirnas were detected and confirmed by stem-loop rt-qpcr in this study. using rnahybrid and pita software, 38 chv-encoded mirnas were predicted to target 41 viral genes and formed a complex regulatory network. dual luciferase reporter assay (dlra) confirmed that viral […]