RNAsnap statistics

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RNAsnap specifications

Information


Unique identifier OMICS_20103
Name RNAsnap
Software type Application/Script
Interface Web user interface
Restrictions to use Academic or non-commercial use
Input data A RNA sequence.
Input format FASTA
Computer skills Basic
Stability Stable
Maintained Yes

Maintainers


  • person_outline Yaoqi Zhou <>
  • person_outline Yuedong Yang <>

Information


Unique identifier OMICS_20103
Name RNAsnap
Software type Application/Script
Interface Command line interface
Restrictions to use Academic or non-commercial use
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes

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Maintainers


  • person_outline Yaoqi Zhou <>
  • person_outline Yuedong Yang <>

Publication for RNAsnap

RNAsnap in publications

 (20)
PMCID: 5918244
PMID: 29649242
DOI: 10.1371/journal.pgen.1007348

[…] of 1:100 into dm25. cells were grown until they reached ~50% of the final od420 and the entire culture (10 ml) was harvested for extracting rna. rna was extracted from frozen cell pellets using the rnasnap protocol []. the resulting supernatant was column purified, incorporating on-column dnase treatment (rna clean & concentrator-25, zymo research). tapestation analysis (agilent) was used […]

PMCID: 5864092
PMID: 29529043
DOI: 10.1371/journal.pgen.1007283

[…] bacterial growth was measured by synergy ht biotek plate reader at 37°c for 55 h. od600 measurements were taken every 15 min after shaking for 2 min., total rna was extracted from bacteria using the rnasnap method []. briefly, bacterial pellets were washed with ae buffer (50 mm naoac ph 5.2, 10 mm edta) and then resuspended in 95% formamide, 18 mm edta, 1% 2-mercaptoethanol and 0.025% sds. […]

PMCID: 5769350
PMID: 29338696
DOI: 10.1186/s12864-017-4413-z

[…] – 0.0017 for all time points) were prepared for rna-seq., one cell pellet from each condition and sampling time was thawed on ice for all three biological replicates; total rna was isolated by rnasnap™ [] and stored at −70 °c. dna contamination was removed by two treatments with turbo-dnase (ambion; life technologies). rna concentrations and a260/a280 ratios were determined using […]

PMCID: 5700377
PMID: 29205229
DOI: 10.1128/mSphere.00483-17

[…] ix 83 inverted microscope with a magnification of ×100. the images were processed with imagej software, and representative images were used., total rna from cells induced for 5 h was isolated using rnasnap rna isolation method as previously described (). the total rna was treated with 1 u/µl dnase (invitrogen). treated rna samples were used to synthesize cdnas by revertaid first strand cdna […]

PMCID: 5636819
PMID: 29021529
DOI: 10.1038/s41598-017-13030-x

[…] 5 ml sample was quickly centrifuged at 16,000 × g in 1.5 ml eppendorf tubes, supernatant removed and pellets flash-frozen in liquid nitrogen and kept on dry ice. total rna was extracted using the rnasnap tm method. pellets were resuspended in 110 μl boil solution (95% [vol/vol] rna-grade formamide, 18 mm edta, 0.025% [wt/vol] sds, 1% 2-mercaptoethanol in ultrapuretm (thermofisher) distilled […]


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RNAsnap institution(s)
Institute for Glycomics and School of Information and Communication Technology, Griffith University, Gold Coast, QLD, Australia; School of Computer Science and Information Engineering, Hefei University of Technology, Hefei, China; Institute of Health and Biomedical Innovation, Queensland University of Technology, Queensland, Australia; Shandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, China
RNAsnap funding source(s)
Supported by grants from the National Natural Science Foundation of China (61271378 and 61671107), the Taishan Scholars Program of Shandong province of China, National Natural Science Foundation of China (61540025), National Health and Medical Research Council (1059775 and 1083450) of Australia, and a National Health and Medical Research Council Early Career Fellowship (1091816).

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