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Protocols

RNAsol specifications

Information


Unique identifier OMICS_32530
Name RNAsol
Interface Web user interface
Restrictions to use None
Input data A nucleotide sequence.
Input format FASTA
Output data A file that includes the accessible surface area and the relative solvent accessibility for each nucleotide in the RNA sequence.
Computer skills Basic
Stability Stable
Maintained Yes

Maintainers


  • person_outline Jianyi Yang
  • person_outline Zhenling Peng

Publication for RNAsol

RNAsol citations

 (7)
library_books

Aryl Hydrocarbon Receptor Antagonists Mitigate the Effects of Dioxin on Critical Cellular Functions in Differentiating Human Osteoblast Like Cells

2018
Int J Mol Sci
PMCID: 5796174
PMID: 29324662
DOI: 10.3390/ijms19010225

[…] ch was performed using a Bio-Rad iQ5 Multicolor Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) in the Equipment Core Facility of the Simpson Querrey Institute at Northwestern University. RNAsol, MinElute columns, complementary DNA (cDNA) synthesis kits, and 2X amfiSure qGreen qPCR Master Mix 2 were purchased by GenDepot (Barker, TX, USA). QPCR was performed using the following program […]

library_books

MLL AF9 and MLL AF4 oncofusion proteins bind a distinct enhancer repertoire and target the RUNX1 program in 11q23 acute myeloid leukemia

2017
Oncogene
PMCID: 5474565
PMID: 28114278
DOI: 10.1038/onc.2016.488

[…] Total RNA was extracted with TRIzol (Invitrogen, Bleiswijk, The Netherlands) or RNAsol (GenDepot, Barker, TX, USA), treated with DNAse on column (Qiagen, Venlo, The Netherlands) and analyzed by reverse transcriptase–qPCR or strand-specific sequencing. […]

library_books

A Parallel G Quadruplex Binding Protein Regulates the Boundaries of DNA Elimination Events of Tetrahymena thermophila

2016
PLoS Genet
PMCID: 4780704
PMID: 26950070
DOI: 10.1371/journal.pgen.1005842

[…] Total RNA (4μg), isolated by RNAsol extraction [], was converted to cDNA using SuperScript II reverse transcriptase as described []. PCR was performed using Lia3rt_FW and Lia3rt_RV primers () to monitor LIA3 expression and HhpI_F […]

library_books

Dual attenuation of proteasomal and autophagic BMAL1 degradation in ClockΔ19/+ mice contributes to improved glucose homeostasis

2015
Sci Rep
PMCID: 4521189
PMID: 26228022
DOI: 10.1038/srep12801

[…] Total RNA was extracted from cells using the PureXtract RNAsol reagent (GenDEPOT) following the manufacturer’s protocol. cDNA was synthesized from 2 μg of total RNA using amfiRivert Platinum cDNA synthesis kit (GenDEPOT). Specific qRT-PCR primers are liste […]

library_books

Anti Bacterial Effects of Poly N Acetyl Glucosamine Nanofibers in Cutaneous Wound Healing: Requirement for Akt1

2011
PLoS One
PMCID: 3084735
PMID: 21559496
DOI: 10.1371/journal.pone.0018996

[…] For semi-quantitative RT-PCR, RNA was extracted with RNAsol (Teltest, Inc.) following manufacturer's instructions. cDNA was synthesized from 2 µg total RNA with a Superscript First Strand Synthesis Kit (Invitrogen), using Oligo(dT) following the manufac […]

library_books

CD205 (DEC 205): A recognition receptor for apoptotic and necrotic self

2009
Mol Immunol
PMCID: 2680960
PMID: 19135256
DOI: 10.1016/j.molimm.2008.11.016

[…] RNA was extracted from a murine thymus and the F1 cell line () using RNAsol B (Biogenesis), according to the manufacturer's instructions. cDNA was synthesised using the First-strand cDNA Synthesis Kit (GE Healthcare) according to the manufacturer's instructions. cDNA e […]

Citations

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RNAsol institution(s)
School of Mathematical Sciences, Nankai University, Tianjin, China; Center for Applied Mathematics, Tianjin University, Tianjin, China
RNAsol funding source(s)
Supported by the National Natural Science Foundation of China (NSFC 11501306 and 11501407), the Fok Ying-Tong Education Foundation (161003), the Fundamental Research Funds for the Central Universities, and the Thousand Youth Talents Plan of China.

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