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Robust Quantitative Scratch Assay RQSA

A software tool for analysis of monolayer wound healing assays. This Robust Quantitative Scratch Assay algorithm introduced statistical outputs where migration rates are estimated, cellular behaviour is distinguished and outliers are identified among groups of unique experimental conditions. Furthermore, the RQSA decreased measurement errors and increased accuracy in the wound boundary at comparable processing times compared to previously developed method (TScratch).

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RQSA classification

RQSA specifications

Software type:
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Programming languages:
Command line interface
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Unix/Linux, Mac OS, Windows
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RQSA distribution


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RQSA support


  • Igor Jurisica <>


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Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada; Princess Margaret Cancer Centre and Techna, University Health Network, Toronto, ON, Canada; Department of Physiology, University of Toronto, Toronto, ON, Canada; Lunenfeld Tanenbaum Research Institute, Toronto, ON, Canada; Department of Obstetrics and Gynecology, University of Toronto, Toronto, ON, Canada; Department of Computer Science, University of Toronto, Toronto, ON, Canada

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