Protocols

Rolling Ball Background Substraction specifications

Information


Unique identifier OMICS_25933
Name Rolling Ball Background Substraction
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Java
Computer skills Advanced
Stability Stable
Requirements
ImageJ
Source code URL https://imagej.net/plugins/download/Rolling_Ball_Background.java
Maintained Yes

Versioning


No version available

Rolling Ball Background Substraction citations

 (2)
library_books

INA complex liaises the F1Fo ATP synthase membrane motor modules

2017
Nat Commun
PMCID: 5665977
PMID: 29093463
DOI: 10.1038/s41467-017-01437-z

[…] ntitative data are presented as mean ± standard error of the mean (SEM). “n” represents the number of biological replicates. Quantifications were performed using ImageQuant TL (GE Healthcare) using a rolling ball background substraction. Number of replicates, controls, and statistical tests are in accordance with published studies employing comparable techniques and are generally accepted standard […]

call_split

Mapping the functional versatility and fragility of Ras GTPase signaling circuits through in vitro network reconstitution

2016
eLife
PMCID: 4775219
PMID: 26765565
DOI: 10.7554/eLife.12435.027
call_split See protocol

[…] reate");}list = getFileList(inputpath);for (i = 0; i < list.length; i++)scott_register(inputpath, outputpath, list[i]);We then corrected for uneven sample illumination or background artifacts using a rolling ball background substraction of 50 pixels. These processed images were subsequently used to analyze the signaling behavior of each bead.To analyze the images, beads were either identified and […]

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