SameSpots

[…] ii (ge healthcare) previously calibrated by a greyscale marker (kodak) and 2d images were captured with labscan 6.00 software (ge healthcare). differential analysis was realized by using progenesis samespots v4.1 software (nonlinear dynamics ltd, newcastle, u.k.) for spot detection, background subtraction, spot volume normalisation and expression. the most representative silver-staining gels […]

[…] was carried out using a typhoon trio (ge healthcare). the scan resolution was 100μm/pixel and the manufacturer’s recommended settings were used. the image analysis was carried out using progenesis samespots software (nonlinear dynamics, version 3.1). the three subcellular fractions were analyzed as independent data sets, each set containing 12 gels and 36 analytical gel images at each of three […]

[…] were stained with colloidal coomassie brilliant blue g-250. to ensure protein pattern reproducibility, three technical replicates were done. the scanned gel images were transferred to the progenesis samespots software package (nonlinear dynamics, newcastle, uk), which allows spot detection, background subtraction, and protein spot od intensity quantification (spot quantity definition). the gel […]

[…] to compare secretomes of js14 and js22. the fluorescent imaging of the 2‐de gels detected a total of 106 and 88 protein spots in the js14‐ and js22‐associated secretomes respectively (fig. a). the samespots analysis identified 14 (in js14) and 13 protein (js22) spots showing at least a twofold (p > 0.05) difference in abundance between the two secretomes (fig. a). the selected protein spots […]

[…] used in phase ii., 2d gel images were captured with typhoon fluorescence imager (amersham) at wavelengths corresponding to each dye. a total of 12 images were analyzed with the software progenesis samespots (nonlinear dynamics). statistical analysis of protein expression levels were determined for each spot based on mean spot volume (n = 12), and differences in protein expression […]

[…] suggested protocol then visualized with biomolecular imagers (fla 7000; life sciences, uk). for spot intensity comparison, isolated omps from three independent replicates were analyzed by progenesis samespots software, uk., the protein bands were excised from either the 10% polyacrylamide gel or the 2d gel electrophoresis second-dimension gel. for in gel digestion, the gel bands or spots […]