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SameSpots specifications


Unique identifier OMICS_02540
Name SameSpots
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes


No version available


  • person_outline SameSpots Team

SameSpots citations


Secretome profiling of Propionibacterium freudenreichii reveals highly variable responses even among the closely related strains

PMCID: 5902329
PMID: 29488359
DOI: 10.1111/1751-7915.13254
call_split See protocol

[…] p was omitted, and two post‐staining rinses with fresh MilliQ water were applied to wash the gel prior to the acquisition of the gel images as described above. The 2‐DE images were analysed using the samespots software v4.5 (Totallab, UK) with four individual gel replicates from both strains. Non‐spot data from the 2‐DE images were first removed by cropping the images, which was followed by automa […]


Leaf Physiological and Proteomic Analysis to Elucidate Silicon Induced Adaptive Response under Salt Stress in Rosa hybrida ‘Rock Fire’

Int J Mol Sci
PMCID: 5578157
PMID: 28805727
DOI: 10.3390/ijms18081768
call_split See protocol

[…] Protein spots from three individual replicates were analyzed by Progenesis SameSpots 2D software v4.1 (Nonlinear Dynamics, Newcastle, UK). Differentially-expressed proteins between the treatments on the spots showed more than 1.5-fold changes (p ≤ 0.05) in one way ANOVA anal […]


Profiling of Campylobacter jejuni Proteome in Exponential and Stationary Phase of Growth

Front Microbiol
PMCID: 5435804
PMID: 28572800
DOI: 10.3389/fmicb.2017.00913

[…] The image analysis was performed using the Progenesis Samespots® software (NonLinear Dynamics). For the statistical analysis of the results, three independent experiments were performed, each with two technical replicates. Differences between the two con […]


Membrane enriched proteome changes and prion protein expression during neural differentiation and in neuroblastoma cells

BMC Genomics
PMCID: 5401558
PMID: 28431525
DOI: 10.1186/s12864-017-3694-6

[…] (w/v) of Coomassie brilliant blue G250 (Amresco).Gels were scanned using a Typhoon Trio Variable Mode Imager (GE Healthcare) at a resolution of 100 μm. The gel images were analysed using the software SameSpots (TotalLab). Spot comparisons between the samples were carried out by calculating the ratio between the average intensity of the conditions. When the average intensity of a spot measured in a […]


Food Like Growth Conditions Support Production of Active Vitamin B12 by Propionibacterium freudenreichii 2067 without DMBI, the Lower Ligand Base, or Cobalt Supplementation

Front Microbiol
PMCID: 5340759
PMID: 28337185
DOI: 10.3389/fmicb.2017.00368

[…] the last rinsing step, the fluorescent gel images were directly captured using the AlphaImagerHP documentation system (ProteinSimple, Santa Clara, California, USA). 2DE images were analyzed using the SameSpots software v4.5 (Totallab, Newcastle, England). Non-spot data from the 2DE images were first removed by cropping the images, which was followed by automatic normalization and selection of the […]


Comparative proteomic analysis of horseweed (Conyza canadensis) biotypes identifies candidate proteins for glyphosate resistance

Sci Rep
PMCID: 5309786
PMID: 28198407
DOI: 10.1038/srep42565
call_split See protocol

[…] ere particularly interested in identifying proteins whose response to glyphosate in the susceptible biotype was blocked in the resistant biotype and vice-versa. The image analysis software Progenesis SameSpots generated ratios from the normalised spot volumes and gave probability values associated with the analysis of variance and Student’s t-tests. Protein spots that were significantly (p ≤ 0.05) […]


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SameSpots institution(s)
TotalLab, Newcastle, USA

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